_____________________________________________________________________________________________________ *Corresponding author E-mail: osuochak@gmail.com; International Journal of Biochemistry Research & Review 31(1): 54-64, 2022; Article no.IJBCRR.83557 ISSN: 2231-086X, NLM ID: 101654445 LC-MS, GC-MS and Hematological Profile of Mucuna pruriens Extracts in Alloxan Induced Diabetic Albino Rat E. C. Chukwu a* , K. U. Osuocha b , B. Musa c and J. C. Njoku d a Department of Biochemistry, Federal University of Lafia, Nasarawa State, Nigeria. b Biochemistry Unit, Department of Science Laboratory Technology, Federal University of Lafia, Nasarawa State, Nigeria. c Centre for Dry Land Agriculture, Bayero University Kano, Nigeria. d Department of Pharmacology, Niger Delta University , Bayelsa State, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IJBCRR/2022/v31i130300 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/83557 Received 15 December 2021 Accepted 25 February 2022 Published 28 February 2022 ABSTRACT Mucuna pruriens also known as velvet beans is an annual climbing shrub that has been claimed traditionally to possess anti anemic potentials. The present study evaluated bioactive constituents and hematological profile of Mucuna pruriens seed and leaf extracts on alloxan induced diabetic male albino rat using standard analytical method. Result of LC-MS investigation on the seed shows the presence of Hydroxystreptozolin ( Oxazolidene) and Dihydrocapsaicin (Meltoxyphe.nol) while the leaf reveals the presence of four(4) bioactive compound: Furocoumurine acid, Epioxylubianin, Acetyllaustoinid and Quassin. The GC-MS studies on Mucuna pruriens seed indicates the presence of twelve bioactive compound. 2-methyl-z,z-3,13-octadecadienol cis-13-octadecadienoic acid, methylester hexadecanoic acid, methylester pentadecanoic acid, 9,12,octadecadienoic acid (z, z)-methylester, 11-octadenoic acid, methylester q-octadecenoic acid, Octadecanamide- N-(2-hydroxyethyl) dodecanamide,N-(2-hydroxyethyl), urea,triethyl-Ethanamine, N, N-dimethyl (phenylmethoxy), 13-Hexyloxacyclotridec carbonitrile,3,5bis[C2dimethylamino]ethylthio, 12- octadecadienoic acid(z, z)-cis-13-octadecenoic acid, 9,12-octadecadienoicacid(z,z)-2-hydroxy- 1(Hydroxymethyl) ethylester, cyclopentadecanane,2-hydroxy-9,12-octadeadienol,LE, etc while GC- Original Research Article