ORIGINAL ARTICLE Bali Medical Journal (Bali Med J) 2019, Volume 8, Number 3 (P2B2 PABI XVI Manado, Special Issue 2019): S799-S802 P-ISSN.2089-1180, E-ISSN.2302-2914 S799 Open access: www.balimedicaljournal.org and ojs.unud.ac.id/index.php/bmj CrossMark Published by DiscoverSys ABSTRACT Background: Gastric perforation covers 25-30% of acute abdomen in the ER and has high mortality and morbidity rate. Surgery is the primary treatment of gastric perforation. The omental patch is commonly used to close the gastric perforation. One method is to use bioabsorbable material glued with fbrin glue. This study aims to determine the efect of bioabsorbable material as a sutureless technique in wound healing of gastric perforation. Method: A true laboratory experimental post-test only control group design using a single-blind technique was conducted among 20 Wistar rats. They were divided into 2 random groups, 10 fbrillar and fbrin glue group and 10 omental patch group. Perforation was made by forming a small hole with a diameter of 0.5 cm using a punch biopsy. The microscopic examination with Hematoxylin-eosin staining was carried out for the number of macrophages and fbroblasts from the gastric perforation site. Data were analyzed using SPSS version 16 for Windows. Result: The average number of fbroblasts produced by the fbrillar and fbrin glue group was 7.5 and 5.4 in omental patch group. Based on the result of T-test (fbroblasts), it was obtained that there was a signifcant diference (H1 is accepted) in the number of fbroblasts produced using fbrillar and fbrin glue compared to the omental patch method (P<0.001). The average number of macrophages in fbrillar and fbrin glue group was 4 and 3 in omental patch group. Based on T-test, it was found a signifcant diference in the number of macrophages produced using fbrillar and fbrin glue compared to the omental patch method (P<0.05) Conclusion: The administration of fbrillar and fbrin glue biomaterials in the closure of gastric perforation increased the number of macrophages and fbroblast cells better than the omental patch method. Keywords: Bioabsorbable, fbrin glue, macrophages, fbroblast, omental patch Cite This Article: Resdiyanto, Niam, M.S., Rasyid, H.A., Sugiharto, S., Inggarwati, L., Susilo, H. 2019. The efect of bioabsorbable (fbrillar) and fbrin glue on the number of macrophages and fbroblasts in Wistar rats with gastric perforation compared to the omental patch method. Bali Medical Journal 8(3): S799-S802. DOI:10.15562/bmj.v8i3.1561 The effect of bioabsorbable (fibrillar) and fibrin glue on the number of macrophages and fibroblasts in Wistar rats with gastric perforation compared to the omental patch method Resdiyanto, 1* Muhammad Shobachun Niam, 1 Harun Al Rasyid, 1 Setyo Sugiharto, 1 Lulik Inggarwati, 1 Hery Susilo 1 INTRODUCTION Gaster is a gastrointestinal organ with essential digestive function, nutrition and endocrine. 1 Tis organ stores and facilitates the digestion and absorption of food and helps appetite regulation. Te gastric disease is a common pathological condi- tion, and gaster is an organ that relatively responds well to therapy. 1 One of the most common gastric conditions that ofen occurs is gastric perforation. 2 Gastric perfo- ration covers 25-30% of acute abdominal diseases in the ER and has a high mortality and morbidity rate. 2 A previous data states that deaths from gastric perforation in the world during 14-year of study reached 5.8% at hospital postoperatively. 3 Gastric perforation is defned as the formation of a hole in the wall of part of the gastrointestinal tract, where the hole allows gastric contents to enter abdominal cavity and cause peritonitis. Te most common aetiology of gastric perforation is ulcers, with an incidence of perforation between 2-10% of all patients with peptic ulcer. 4 Other causes of gastric perforation are trauma, neoplasms, ingestion of foreign and iatrogenic objects. 4 Currently, the incidence of gastric perforation is increasing in the elderly population (60-70 years). It is ofen associated with NSAID use in the elderly population. 5 Perforation develops into chemical peritonitis due to gastric acid leakage into the abdominal cavity, and within a few hours, bacte- rial contamination occurs. Gastric perforation is a life-threatening condition, so that early diagnosis and treatment shall be immediately performed. 4,5 Te treatment for gastric perforation cases is primarily surgery. 6 But the real truth on the ground, not all cases of gastric perforation can be immediately performed by defnitive surgery, for example in septic shock condition. Defnitive procedures are delayed until the patient’s condition 1 Department of Surgery, Saiful Anwar General Hospital, Malang, Indonesia * Correspondence to: Resdiyanto; Department of Surgery, Saiful Anwar General Hospital, Malang, Indonesia; resdiyanto@gmail.com Received: 2019-07-17 Accepted: 2019-11-14 Published: 2019-12-01