Interactions of the LIPG 584C O T polymorphism and alcohol consumption on serum lipid levels Wan-Ying Liu a,b , Rui-Xing Yin a, * , Lin Zhang a , Dong-Feng Wu a , Lynn Htet Htet Aung a , Xi-Jiang Hu a , Xiao-Li Cao a , Lin Miao a a Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P. R. China b Department of Cardiology, The Third Affiliated Hospital (The Second People’s Hospital of Nanning), Guangxi Medical University, Nanning, Guangxi 530031, P. R. China Received 1 November 2010; received in revised form 11 July 2011; accepted 11 July 2011 Abstract Both endothelial lipase gene (LIPG) 584C O T (rs2000813) polymorphism and alcohol consumption modulate serum lipid levels. But their interactions on serum lipid profiles are not well known. The present study was undertaken to detect the interactions of LIPG 584C O T polymorphism and alcohol consumption on serum lipid levels. Genotyping of the LIPG 584C O T was performed in 763 unrelated nondrinkers and 520 drinkers aged 15e85 years. The levels of serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), apolipoprotein (Apo) AI, and the ratio of ApoAI to ApoB were higher in drinkers than in nondrinkers (P ! .01 for all). There were no significant differences in the genotypic and allelic frequencies between nondrinkers and drinkers. The levels of TC, HDL-C, and ApoAI in nondrinkers were different among the three genotypes (P ! .05e.01), the subjects with CT genotype had higher TC, HDL-C, and ApoAI levels than the subjects with CC genotype. The levels of HDL-C and ApoAI in drinkers were different among the three genotypes (P ! .001 and P ! .05; respectively), the individuals with TT genotype had higher HDL-C and ApoAI levels than the individuals with CT and CC genotypes. The interactions between LIPG 584C O T genotypes and alcohol consumption on serum HDL-C (P ! .01) and ApoAI levels (P ! .05) were also detected by using a factorial regression analysis after controlling for potential confounders. The levels of TC in nondrinkers were correlated with LIPG 584C O T alleles (P ! .05), whereas the levels of TG and HDL-C were associated with LIPG 584C O T alleles (P ! .05) and genotypes (P ! .05), respectively. These results suggest that the subjects with TT genotype benefit more from alcohol consumption than the subjects with CT and CC genotypes in increasing serum HDL-C and ApoAI levels. Ó 2011 Elsevier Inc. All rights reserved. Keywords: Endothelial lipase gene; Polymorphism; Alcohol consumption; Lipids; Apolipoproteins; Interaction Introduction Despite major advances in treatment, coronary artery disease (CAD) remains the biggest cause of mortality and morbidity in the developed world (Yusuf et al., 2001). Epidemiological studies have shown that low levels of high-density lipoprotein cholesterol (HDL-C) are closely related to the development and progression of atheroscle- rosis and a higher incidence of CAD (Gordon et al., 1989; Sharrett et al., 2001). Cumulative evidences show that an increase of 1 mg/dL of HDL-C levels is associated with a 2e3% decrease of the risk for CAD in men and women, respectively (Linsel-Nitschke and Tall, 2005; Wilson, 1990). The regulation of HDL-C levels is a compli- cated and incompletely understood process, and is likely to depend on the interactions of both environmental and genetic factors. Several genetic polymorphisms in lipid metabolism-related genes have been shown to interact with alcohol consumption on serum lipid concentrations (Corella et al., 2001; Gudnason et al., 1997; Ruixing et al., 2010; Wang et al., 2004; Yin et al., 2011; Zhou et al., 2008). Both caseecontrol and cohort studies have described a J- or U-shaped association between alcohol intake and CAD (Kiechl et al., 1998; McElduff and Dobson, 1997) and between alcohol intake and all-cause mortality (Grønbaek et al., 1998). Low to middle amounts of alcohol when taken on a regular basis have been shown to protect * Corresponding author. Department of Cardiology, Institute of Cardiovascular Diseases, the First Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning, Guangxi 530021, P. R. China. Tel.: þ86-771-5326125; fax: þ86-771-5353342. E-mail address: yinruixing@yahoo.com.cn (R.-X. Yin). 0741-8329/$ - see front matter Ó 2011 Elsevier Inc. All rights reserved. doi: 10.1016/j.alcohol.2011.07.002 Alcohol 45 (2011) 681e687