JEMAT, 2019, Vol 7, No 1, 27-31 - 27 - Arrhenius Plot Analysis, Temperature Coefficient and Q10 Value Estimation for the Effect of Temperature on the Growth Rate on Acrylamide by the Antarctic Bacterium Pseudomonas sp. Strain DRYJ7 Aa'ishah Abd Gafar 1 , Motharasan Manogaran 1 , Nur Adeela Yasid 1 , Mohd Izuan Effendi Halmi 2 , Mohd Yunus Shukor 1 and Ahmad Razi Othman 3 * 1 Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia. 2 Department of Soil Management, Faculty of Agriculture Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia. 3 Department of Chemical Engineering, Faculty of Engineering and Build Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, D.E, Malaysia. *Corresponding author: Dr Ahmad Razi Othman Department of Chemical Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, D.E, Malaysia. Email: ahmadrazi@ukm.edu.my INTRODUCTION The majority of microbes, because of their minute size are strongly influenced by the ambient temp, as they are ectothermic. The outcome of temperature fluctuations influences microorganism adaptation and physiology via the modulation of their biochemical pathway. For this reason, temperature is certainly an important aspect that must be taken into account when understanding the biodegradation of toxic chemicals by microorganisms. The Arrhenius version is extremely popular to understand the actual end result of the effect of temperature in regards to the growth of harmful bacteria and has been utilized for the approximation of the apparent activation energy or ∆H*, for growth or degradation on toxic substances. The values are HISTORY Received: 11 th Feb 2019 Received in revised form: 17 th of May 2019 Accepted: 17 th of June 2019 ABSTRACT Several models are available to model the effect of temperature on the growth rate of microorganism on substrates. One of the models is Arrhenius and is very popular due to it having few parameters. An apparent activation energy based on the Arrhenius plot on the growth on acrylamide by Pseudomonas sp. strain DRYJ7 on acrylamide is reported for the first time. The bacterium was grown in minimal salts media supplemented with 1000 mg/L of acrylamide as a nitrogen source and glucose as the carbon source. The plot of ln ฀m (specific growth rate) against 1/T for growth on acrylamide was carried out spanning the range of temperature from 10 to 35 o C. Regression analysis from 10–20 o C results in an activation energy of 14.96 kJ mol -1 . A relative 10 ˚C increase in the surrounding temperature, usually results in doubling the reaction rate, with corresponding Q10 value of 1.8, which is the approximate value for a number of biological reactions. The Q10 values, determined from the Arrhenius plot of 2.17 and a theta value of 1.03 obtained in this work, are within the normal range for many biological values. This is the first time that values for the activation energy, Q10 and theta for the growth of a bacterium on acrylamide is reported. KEYWORDS Antarctica; Acrylamide-degrading; temperature; Pseudomonas sp.; Arrhenius plot JOURNAL OF ENVIRONMENTAL MICROBIOLOGY AND TOXICOLOGY Website: http://journal.hibiscuspublisher.com/index.php/JEMAT/index JEMAT VOL 7 NO 1 2019