Research Article Elucidation of the Effects of Si-Wu Tang on Menstrual Disorder Patterns through Activation of Aromatase and Antioxidation Guan-Cheng Huang, 1,2 Yi-Zhe Tsai, 3 Chia-Jung Lee, 4,5 Heng-Yu Chang, 6 and Ching-Chiung Wang 3,4,5,7 1 Division of Hemato-Oncology, Department of Internal Medicine, Yuan’s General Hospital, Kaohsiung City 802, Taiwan 2 Program of Health-Business Administration, School of Nursing, Fooyin University, Kaohsiung City 831, Taiwan 3 Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan 4 PhD Program for Clinical Drug Discovery of Chinese Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan 5 Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 110, Taiwan 6 School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 7 School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan Correspondence should be addressed to Ching-Chiung Wang; crystal@tmu.edu.tw Received 22 November 2018; Revised 7 February 2019; Accepted 21 February 2019; Published 5 March 2019 Academic Editor: Svein Haavik Copyright © 2019 Guan-Cheng Huang et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Si-Wu Tang (SWT), a traditional Chinese formula, is commonly used for treating female diseases, such as relief of menstrual discomfort and climacteric syndrome. Te aim of this study was to explore the synergistic efects between each herb in SWT on menstrual disorder patterns. Estradiol regulation and antioxidative efects were indicators that ameliorated menstrual disorder patterns and the total polyphenol and polysaccharide contents were quality markers. According to relationships of bioactivity and phytochemical contents, we discuss the efects of each herb in SWT. In a testosterone-treated MCF-7 cell model, Rehmannia glutinosa and catalpol signifcantly increased the estradiol content and aromatase upregulation in cell culture. We suggest that catalpol is an aromatase promoter in SWT, and R. glutinosa is a major actor. In terms of the antioxidant activity, pentagalloylglucose, gallic acid, and ferulic acid had stronger antioxidative efects than other compounds. We suggest that the antioxidative ability depends on polyphenols, and Paeonia lactifora is a major contributor. Based on the prescribing principle of traditional Chinese medicine (TCM) theory, we suggest that R. glutinosa in SWT act as an aromatase promoter in the role of sovereign for ameliorating menstrual disorder patterns. As P. lactifora has the strongest antioxidant efects and can prevent ROS damage ovarian; therefore, P. lactifora could help R. glutinosa work as a minister for menstrual disorder patterns and R. glutinosa and P. lactifora are a herbal pair in SWT. 1. Introduction Si-Wu-Tang (SWT), a traditional Chinese formula consisting of Rehmanniae Radix (the processed root of Rehmannia glutinosa Libosch, Shu Di Huang ), Angelica Radix (the root of Angelica sinensis Diels, Dang Gui), Chuanxiong Rhizoma (the rhizome of Ligusticum chuanxiong Hortorum, Chuan Xiong ), and Paeoniae Radix (the root of Paeonia lactifora Pall, Shao Yao), was frst recorded in Tai Ping Huei Min Ho Chi Chu Fang of the Sung dynasty (A.D. 1107∼1110). It has traditionally been used for treating gynecological diseases, such as relief of menstrual irregularity, dysmen- orrhea, uterine bleeding, climacteric syndrome, and other estrogen-related diseases. Furthermore, traditional medicine doctors also use it to cure weakness in various parts of the body due to anemia [1]. Recently, there have been many Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2019, Article ID 4761651, 8 pages https://doi.org/10.1155/2019/4761651