Research Article Open Access Mabrouk et al., Bioceram Dev Appl 2013, S:1 DOI: 10.4172/2090-5025.S1-009 ISSN: 2090-5025 BDA, an open access journal Bioceram Dev Appl Conferences Proceedings: ISACB-6 Keywords: Tissue engineering; Scafolds; Ciprofoxacin; Drug release; Freeze drying; Polyvinyl alcohol Introduction Bioactive glass (BG) has various applications in repair and reconstruction of bone tissue; however, it has week mechanical properties especially in porous form. One approach to enhance the mechanical properties of materials is the elaboration of BG with polymer to form composites [1]. Tis way leads to an excellent combination between strength and toughness, as well as improved characteristics, when compared to their individual components [2]. Te composites of BG/polymer are able to provide construct with excellent osteogensis and angiogenesis [1]. Application of a drug to a specifc region using drug loaded scafold produce high concentration of the drug in the required site of action which eliminate the side efects that prohibit the administration of large oral dose. Ciprofoxacin (Cip) is a furoquinolone derivative, widely used as an antibiotic and in osteomyelitis because of its favorable penetration and bactericidal efect on all the probable osteomyelitis pathogens [3]. Te main purpose of the current study was to develop and fabricate a construct of bioactive scafold combining an antibiotic (ciprofoxacin) as a target drug delivery system. Characterization of these scafolds before and afer addition of the drug has been investigated by XRD, FTIR, mercury porosimeter, SEM, universal testing machine and UV-spectrophotometer. Materials and Methods Synthesis Te 46S6 bioactive glass was prepared as previously reported [4] by using calcium silicate (Alfa Aesar), trisodium trimeta phosphate and sodium metasilicate pentahydrate (Sigma). Te prepared glass was named MB. Polyvinyl alcohol (PVA) and polyvinyl alcohol/bioactive glass in molar ratio 1:2 PVA/MB scafolds and scafolds loaded with ciprofoxacin drug (PVA/MB-cip) were prepared by employing thermally induced phase separation technique (freeze drying). Firstly, 15% of PVA (Aldrich, M. wt= 67.000) was dissolved in distilled water at 80°C for 2 hr. Te prepared glass MB was added to polymer solution with continuous stirring at room temperature for 20 hr. Ten, exactly 5, 10 and 20% of ciprofoxacin drug were added to the batch of PVA and to that of PVA/MB composite with stirring for 1 hr. Afer that, the mixture was poured in moulds to form cylinders with diameters of 15 mm × 10 mm and kept at -18°C overnight, then transferred to a freeze dryer type Christ, Alpha 1-2 LD plus for 24 hr. Characterizations Te porosity and the micro architecture of the prepared scafolds were assessed by means of Mercury Intrusion Porosimetry (MIP) by using Poresizer 9320 V2.08, liquid displacement method and scanning electron microscopy (SEM) (Jeol JSM 6301).Te phases and structure of each components of the prepared scafold had been examined by XRD (Philips X-PERT with Cu Kα wave length of 1.5418 Å) and FTIR (Nicolet Magna-IR 550). Compressive strength was measured by universal testing machine type Lloyd. Te release of the drug was constructed in PBS at pH 7.4 and drug concentration was followed using UV-spectrophotometer (Jenway 6705) at 277 nm. Results and Discussion Morphological and microstructural properties Te morphology of the prepared scafolds is presented in (Figure *Corresponding author: AA Mostafa, University of Rennes 1, UMR CNRS 6226, 263 av. du général leclerc, 35042 Rennes Cedex, France, E-mail: amany.mostafa@univ-rennes1.fr Received May 13, 2013; Accepted May 30, 2013; Published September 10, 2013 Citation: Mabrouk M, Mostafa AA, Oudadesse H, Mahmoud AA, Gaafar AM, et al. (2013) Fabrication, Characterization and Drug Release of Ciprofoxacin Loaded Porous Polyvinyl Alcohol/Bioactive Glass Scaffold for Controlled Drug Delivery. Bioceram Dev Appl S1: 009. doi: 10.4172/2090-5025.S1-009 Copyright: © 2013 Mabrouk M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Composite scaffolds of polyvinyl alcohol (PVA) and/or quaternary bioactive glass (46S6 system) containing 5, 10 and 20 wt % ciprofoxacin were prepared by lyophilisation technique. The porosity of the prepared scaffolds was measured by liquid displacement, Hg-porosimeter and SEM. The structure and the nature of chemical bonds between atoms were examined by XRD and FTIR. They confrmed the incorporation of ciprofoxacin into the scaffolds. Biodegradation rate and drug release behaviour were conducted in phosphate buffer saline (PBS) at pH 7.4. A porous scaffold has been obtained with porosity up to 85%. By increasing the glass contents and drug concentration in the prepared scaffold the porosity and the degradation rate decrease however, the compressive strength was enhanced. A sustained drug release pattern was observed from the optimized scaffold with a quasi-Fickian diffusion mechanism and it was able to deliver the drug in a prolonged release pattern which offers a distinguish treatment for osteomylitis as well as local antibacterial effect. Fabrication, Characterization and Drug Release of Ciprofloxacin Loaded Porous Polyvinyl Alcohol/Bioactive Glass Scaffold for Controlled Drug Delivery M Mabrouk 1,2 , AA Mostafa 1,2 *, H Oudadesse 1 , AA Mahmoud 3 , AM Gaafar 4 and MI El-Gohary 5 1 University of Rennes 1, UMR CNRS 6226, 263 av. du général leclerc, 35042 Rennes Cedex, France 2 Biomaterials Department, National Research Centre, Cairo, Egypt 3 Pharmaceutical Technology National Research Centre (NRC), Cairo, Egypt 4 Photo Chemistry Department, National Research Centre (NRC), Cairo, Egypt 5 Physics Department, Faculty of Science, Al -Azhar University, Cairo, Egypt Bioceramics Development and Applications B i o c e r a m i c s D e v e lo p m e n t a n d A p p l i c a t i o n s ISSN: 2090-5025