of patients with a pathogenic (class 5) variant ranges from 4% if scoring 5 or less to 76% if scoring 15 or more. LDL-C is the strongest discriminatory factor. In relatives of mutation positive FH patients we observed that LDL-C in- creases with age, but this is not taken into account within the criteria. In 250 relatives of mutation positive index patients we observed that geno- type (p<0.001) and age (p<0.001), but not gender, significantly predicted LDL-C in relatives aged 18-70 years (LDL-C ¼ 2.004 + 3.620(genotype 0,1) + 0.035(age)). Applying this effect of age to index patients would suggest that older patients are more likely to have LDL-C high enough to meet the scoring criterion for LDL-C, but younger patients might not because their youth means that their LDL-C may be below the threshold, despite being high for their age. We conclude that the current criteria have proven utility for selecting patients for genetic testing. However there is scope for further refinement to take into account the variation in LDL-C with age. 7. A POSITIVE EXPERIENCE BUT A NEGATIVE RESULT IN SCREENING FOR FAMILIAL HYYPERCHOLESTEROLAEMIA (FH) IN PRIMARY CARE Z. Jayne * , J. Lungley, D. Harvey, D.R. Nair. Department of Clinical Biochemistry, Royal Free London NHS Foundation Trust, London, UK Background: In 2015 the Royal Free Hospital, London, commenced a screening programme to determine whether specialist FH nurses working in primary care can improve the identification of FH. The initial results from one GP practice were promising with the increased identification of possible FH rising from 0.2% to 0.35%. This was in line with the Medway audit, however genetic testing, imperative to provide a firm diagnosis for cascade testing, did not support the evidence. In the second phase a further four GP practices, (30 000 patients) in three CCG’s, were screened, with a fifth currently in progress. Method: The GP database was interrogated to find patients with a read code of FH or family history of FH, those with a TC >7.5mmol/L or LDL-C >4.9mmol/L and with triglycerides <3mmol/L, and those with a history of premature cardiovascular disease. A total of 686 patients met one parameter; following telephone assessment 110 were seen in clinic. Results: A total of 30 patients who met the Simon Broome criteria were referred for genetic testing. Two have a confirmed mutation, 16 did not and 12 results are outstanding. So far the results show the Welsh genotyping score to be 44% accurate and the Simon Broome 11%. No one search proved more effective at identifying indexes. Conclusion: The use of specialist FH nurses in primary care to identify possible FH index cases involved a total of 35 nurse days, this is an important consideration when commissioning for value. Using the prevalence of 1:500 we would have expected to find 60 indexes. Regional demographics may account for our lower preva- lence, the mobile 18-40 age group made up 40% of our screening pop- ulation, of which over 80% had not had a cholesterol test so were not included. This young, mobile population also makes cascade testing less fruitful. 8. IMPROVEMENT IN SMALL FIBRE NEUROPATHY AND INFLAMMATORY BIOMARKERS AFTER BARIATRIC SURGERY Tarza J. Siahmansur 1, * , Yifen Liu 1 , Shazli Azmi 1 , Maryam Ferdousi 1 , Philip Pemberton 2 , Safwaan Adam 1, 3 , Jonathan D. Schofield 1,3 , Akheel A. Syed 4 , Basil Ammori 4 , Rayaz Malik 1 , Paul N. Durrington 1 , Handrean Soran 1, 3 . 1 Cardiovascular Research Group, University of Manchester, Manchester, M13 9NT, UK; 2 Department of Clinical Biochemistry, Central Manchester University Hospitals, NHS Foundation Trust, Manchester, M13 9WL, UK; 3 Cardiovascular Trials Unit, University Department of Medicine, Central Manchester and Manchester Children University Hospital NHS Foundation Trust, Manchester, M13 9WL, UK; 4 Department of Obesity Medicine, Salford Royal Hospital NHS Foundation Trust, Stott Lane, Salford, M6 8HD, UK Aim: To assess for an improvement in small-fibre neuropathy (SFN) and cardiovascular risk markers following bariatric surgery. Methods: Corneal confocal microscopy (CCM) was performed in 22 morbidly obese subjects (non-diabetic (n¼9), T2DM (n¼13)) and mea- surements of corneal nerve fibre density (CNFD), branch density (CNBD) and fibre length (CNFL) were done. In addition, fasting venous blood was taken to assess lipid parameters, oxidative stress and inflammatory biomarkers, at baseline and 12 months after bariatric surgery. Results: Compared to baseline, 12 months after bariatric surgery there were significant improvements in HbA1c, triglycerides, high density li- poproteins (HDL-C), body mass index (BMI), CNFD, CNFL, highly sensitive C-reactive protein (hs-CRP), Interleukin-6, Intercellular Adhesion Mole- cule-1 (ICAM-1), serum amyloid A (SAA), and myeloperoxidase (MPO). Ă Abstracts / Atherosclerosis 255 (2016) e6ee9 e8