Clinopodium odorum (Griseb.) Harley (Lamiaceae) [synonymy: Satureja odora (Griseb.) Epling] is a shrub of up to 2 meters tall and is native to Bolivia and Argentina. It has a pleasant aroma and grows wild in the mountain range of central Argentina. Tis shrub is also known as “muña-muña”, “salviolora” or “peperina”, and is found in undisturbed sites of mountain slopes, at between 1500 and 2000 m.a.s.l [1]. Vázquez AM 1 , Goleniowski ME 2* , Aimar ML 3 , Diaz MS 2 , Demmel GI 1 , Decarlini MF 1 , and Cantero JJ 4 1 Laboratory of Chemical Technology, Faculty of Chemistry, Catholic University of Cordoba, Cordoba, Argentina. Av. Armada Argentina 3555 2 Ceprocor, Science and Technology Ministry, Arenales 230.Córdoba, Argentina 3 Cathedra of Applied Chemistry, Department of Chemistry, Faculty of Exact, Physical and Natural Sciences, National University of Cordoba, Argentina 4 Department of Agricultural Biology, Faculty of Agriculture and Veterinary, University of Río Cuarto, Cordoba, Argentina * Corresponding author: Goleniowski ME, 2Ceprocor, Science and Technology Ministry, Arenales, 230.Córdoba, Argentina, Tel: 00543514681174, E-mail: mgoleniowski@gmail.com Citation: Vázquez AM, Goleniowski ME, Aimar ML, Diaz MS, Demmel GI, et al. (2018) Profle Characterization of Volatile Organic Compounds on In Vitro Propagated Plants of Clinopodium Odorum and it’s Comparison with the Wild Plant. SAJ Pharma Pharmacol 5: 103 RESEARCH ARTICLE Volume 5 | Issue 1 ScholArena | www.scholarena.com ISSN: 2375-2262 Open Access Introduction Abstract Keywords: Tissue culture; Volatile organic compounds; HS-SPME/GC-MS; Menthone; Pulegone SAJ Pharmacy and Pharmacology Profle Characterization of Volatile Organic Compounds on In Vitro Propagated Plants of Clinopodium Odorum and it’s Comparison with the Wild Plant Te aim of this work was to study the efect of the in vitro culture conditions of plants of Clinopodium odorum on the production of volatile organic compounds (VOCs), and to identify any diferences with plants that grow wild. Plantlets were grown with diferent concentrations and combinations of growth regulators and diferent media conditions, with or without plant hormones. Te VOC profles of wild and in vitro cultivated plants were determined by HS-SPME/GC-MS, and the results were interpreted by performing statistical tests (principal component analysis (PCA) and conglomerate analysis (CA)). Tere was a wide variability in the VOC compositions depending on the growing conditions, with (-)-Menthone and Pulegone being the predominant VOCs produced in the most of the media studied. However, Pulegone showed a higher sensitivity to the nutrient composition media, with biosynthesis being favored by low salt concentrations. Moreover, pulegone was accumulated at higher concentrations on Murashige and Skoog (MS) and Gamborg (B5) media at half-strength salt media (MS 1⁄2 and B 51⁄2 ), which was dependent on the PGRs and nutrient addition. In conclusion, our present study shows that the micropropagation of C. odorum through in vitro axillary shoot proliferation is a reliable method for the rapid multiplication of this species, which allows the production of VOCs such as those found in spontaneous plants. In this way, we were able to select the most suitable chemotype. List of abbreviations: VOCs: Volatile Organic Compounds; HS-SPME: Head Space-Solid Phase Micro Extraction; GC-MS: Gas Chromatography Coupled Mass Spectrometry; KI: Kovat´S Retention Indices; PCA: Principal Component Analysis; CA: Conglomerated Analysis; ANOVA: Analysis of Variance; MS: Murashige-Skoog medium; B5: Gamborg medium; SH: Schenk and Hildebrandt medium; WP: Lloyd and Mc Cown medium; PGRs: Plant Growth Regulators; BA: Benzyladenine; GA 3 : Gibberellic Acid; IBA: Indole-3-Butyric Acid; BAP: Benzylaminopurine; NAA: 1-Naphthaleneacetic Acid; IAA: Indoleacetic Acid Te fresh herb is used as a favouring agent in food, and has gained considerable importance in traditional medicine of the local inhabitants for being anticatarrhal, antispasmodic, stringent, carminative, diuretic, stomachic, anti-oxidant, antiacidic, and Article history: Received: 30 November 2017, Accepted: 23 April 2018, Published: 25 April 2018