Volume 7 • Issue 2 • 1000239
Cardiovasc Pharm Open Access journal
ISSN: 2329-6607
Open Access
Tsenum, Cardiovasc Pharm Open Access 2018, 7:2
DOI: 10.4172/2329-6607.1000239
Research Article Open Access
Cardiovascular Pharmacology: Open Access
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ISSN: 2329-6607
Keywords: Diabetes; Hyperlipidemia; Solanum incanum; Glibenclamide;
Alloxan monohydrate
Introduction
Background of the study
Diabetes mellitus (DM) is a metabolic disorder resulting from a
defect in insulin secretion, insulin action, or both. Insulin defciency in
tum leads to chronic hyperglycemia with disturbances of carbohydrates,
fat and protein metabolism [1]. Experimental diabetes in animals
provided a considerable insight into the physiologic and biochemical
derangements of the diabetes state. Studies have shown that individuals
with diabetes have a higher incidence of liver and kidney function
abnormalities, as well as formation of free radicals due to glucose
oxidative, non-enzymatic glycosylation of proteins and subsequent
oxidative degradation of glycated proteins, leading to a decline in
antioxidant defense mechanisms and damage of cellular organelles
and enzymes, increased lipid peroxidation and development of insulin
[2]. It is a long term chronic condition that causes high blood sugar
levels. It is one of the common metabolic disorders with micro and
macrovascular complications that results in signifcant morbidity and
mortality. Diabetes mellitus results from defects in insulin secretion,
insulin sensitivity or both and include a group of metabolic disorders
characterized by hyperglycemia and abnormalities in carbohydrates,
fats and protein metabolism [3]. It is considered as one of the fve
leading causes of death in the world [4,5]. It is recognized as a global
epidemic by the world health organization [6].
Diabetes mellitus occur throughout the world and research has
shown that its prevalence was higher and common in developed
countries than in the developing countries in the mid ‘90s [7]. Globally
as of 2010, an estimated 285 million people had diabetes [8]. In 2013,
according to International Diabetes Federation, an estimated 381
million people had diabetes [9]. Its prevalence is increasing rapidly and
Antihyperlipidemic Effect of Solanum incanum on Alloxan Induced Diabetic
Wistar Albino Rats
Tsenum JL*
Makurdi College of Sciences, Federal University of Agriculture, Abuja, Nigeria
Abstract
The effect of orally administered aqueous fruit extract of Solanum incanum on serum lipid profle of Wistar
Albino rats were determined. Twelve male and female Wistar Albino rats were randomly assigned into four groups of
three rats each, following acclimatization to laboratory and handling conditions. Diabetes was induced with a single
dose of alloxan (120 mg/kg) body weight and plasma glucose was taken 72 h after induction to confrm diabetes.
The normal control was not induced. Animals in group a (normal control) and B (diabetic) were administered 0.5 ml
of normal saline respectively. Group C was administered with 10 mg/kg weight of glibenclamide and group D was
administered 500 mg/kg body weight of aqueous Solanum incanum extract. Extract administration lasted for fourteen
days. Water and feeds were allowed ad libitum. After the two weeks treatment with the plant extract, blood samples
were collected by cardiac puncture for lipid profle analysis by standard methods and enzyme kits. At the end of week
two, the lipid profle of all groups were signifcantly different. The result on lipid profle showed that the extract treated
group was signifcantly lower (P>0.05) in TC, TAG and VLDL as compared to diabetic control but signifcantly higher
(P<0.05) in HDL and LDL as compared to diabetic control. The glibenclamide treated group was signifcantly lower
(P>0.05) in TAG, I-ID L, and VLDL as compared to the diabetic control but signifcantly higher (P<0.05) in TC and
LDL as compared to diabetic control. However, the extract was observed to have a high signifcant level of TC, TG,
HDL, LDL and VLDL compared to the Normal control. Result on weight showed a signifcant difference at week and
week 2. Antihypercholesterolemic and antihypertigliceridemic effect were observed in this result. Results suggest
that Solanum incanum has hypolipidemic effect and therefore makes it benefcial in the dietary management of
cardiovascular complications associated with diabetes and that its consumptions are safe for humans.
*Corresponding author: Joseph Luper Tsenum, Makurdi College of Sciences,
Federal University of Agriculture, Abuja, Nigeria, Tel: +2348160457248/
+2347058245055; Fax: 08160457248; E-mail: tsenumjosephluper@gmail.com
Received April 17, 2018; Accepted April 25, 2018; Published April 30, 2018
Citation: Tsenum JL (2018) Antihyperlipidemic Effect of Solanum incanum on
Alloxan Induced Diabetic Wistar Albino Rats. Cardiovasc Pharm Open Access 7:
239. doi: 10.4172/2329-6607.1000239
Copyright: © 2018 Tsenum JL. This is an open-access article distributed under
the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and
source are credited.
by 2030, this number is estimated to almost double [10]. It is worry
some to note that the major part of this numerical increase is expected
to occur in developing countries where there is rapid urbanization,
nutrition transition and increasingly sedentary lifestyles [11]. Patients
with uncontrolled diabetes mellitus usually experience heart failure
which indicates that hyperglycemia maybe responsible for the disease
[12]. Hyperglycemia produces symptoms of polyuria, polydipsia and
polyphagia. It is also associated with long term damage and failure
of various organs such as eyes, kidney, liver, nerves, heart and blood
vessels. Diabetes mellitus is associated with alteration in the plasma
lipid and lipoprotein profle [13].
Current interest in the search for appropriate agents in the
management of diabetes is therefore focused on traditional medicinal
plants. Natural herbs are plants or plants part used for its scent, favor or
therapeutic properties. Herbal medicines maintain and improve health
and they do not go through the testing that chemotherapeutic agents
go through. Secondly, the plants by means of secondary metabolism,
contain a variety of phytochemical ingredients that are thought to
act on a variety of targets by various modes and mechanisms [14] as
expected from its pathogenicity. Unlike chemotherapeutic agents,
however, herbal remedies have been reported to be nontoxic, accessible