Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Sun, 04 Aug 2019 20:09:37 Taxonomic description and genome sequence of Bacillus campisalis sp. nov., a member of the genus Bacillus isolated from a solar saltern Rajendran Mathan Kumar, 1 3 Gurwinder Kaur, 1 3 Anand Kumar, 1 Monu Bala, 1 Nitin Kumar Singh, 1 Navjot Kaur, 1 Narender Kumar 2 and Shanmugam Mayilraj 1 Correspondence Shanmugam Mayilraj mayil@imtech.res.in 1 MTCC – Microbial Type Culture Collection & Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India 2 Division of Protein Science & Engineering, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India The taxonomic position of a Gram-stain positive bacterium isolated from a solar saltern sample collected from Kanyakumari, coastal region of the Bay of Bengal, India, was analysed by using a polyphasic approach. The isolated strain, designated SA2-6 T , had phenotypic characteristics that matched those of the genus Bacillus. The 16S rRNA gene sequence (1493 bases) of the novel strain was compared with those of previously studied Bacillus type strains and confirmed that the strain belongs to the genus Bacillus and was moderately closely related to the type strain of Bacillus foraminis at 97.5 % 16S rRNA gene sequence similarity, followed by those of Bacillus thioparans (96.9 %), Bacillus subterraneus (96.8 %), Bacillus jeotgali (96.6 %), Bacillus selenatarsenatis (96.6 %) and Bacillus boroniphilus (96.6 %). 16S rRNA gene sequence analysis indicated that strain SA2-6 T differs from all other species of the genus Bacillus by at least 2.5 %. It contained MK-7 as the predominant menaquinone, meso- diaminopimelic acid as the diagnostic cell-wall diamino acid, and iso-C 15 : 0 and anteiso-C 15 : 0 as major fatty acids. Major lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). Based on data from this polyphasic study, strain SA2-6 T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus campisalis sp. nov. is proposed. The type strain is SA2-6 T (5MTCC 11848 T 5DSM 28801 T ). The draft genome of strain SA2-6 T consisted of 5 183 363 bp with G+C content of 45.44 mol%, 5352 predicted coding sequences, 191 RNAs and 479 subsystems. The genus Bacillus comprises Gram-positive, aerobic, spore-forming, taxonomically very diverse rods that have been isolated from different saline habitats such as salterns, estuarine water, salt lakes, salty foods, sea ice and deep-sea hydrothermal vents (Ventosa et al., 1989; Nielsen et al., 1994; Agnew et al., 1995; Arahal et al., 1999; Yoon et al., 2004). Halophilic bacteria are characterized by optimal growth in media containing 3–15 % (w/v) NaCl (Ventosa et al., 1998). They are attracting increasing interest because this group of bacteria has great biotechnological potential for the production of compatible solutes or hydrolytic enzymes (Margesin & Schinner, 2001). Based on molecular and chemical analyses, Gram-positive halophilic bacteria originally assigned to the genus Bacillus have been reclassi- fied as members of novel genera (Heyndrickx et al., 1998; Schlesner et al., 2001; Spring et al., 1996; Stackebrandt & Liesack, 1993; Wainø et al., 1999; Yoon et al., 2001, 2002). The results of 16S rRNA gene sequence analysis have shown that the genus Bacillus contains six phylogenetically distinct groups and that many alkaliphilic/halophilic bacilli belong to rRNA Bacillus group 6 (Nielsen et al., 1994). Some of the moderately halophilic species belonging to the genus Bacillus are Bacillus aurantiacus (Borsodi et al., 2008), Bacillus chagannorensis (Carrasco et al., 2007a), Bacillus coahuilensis (Cerritos et al., 2008), Bacillus isabeliae (Albuquerque et al., 2008), Bacillus oshimensis (Yumoto et al., 2005), Bacillus polygoni (Aino et al., 2008), Bacillus 3These authors contributed equally to this paper. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SA2-6 T is AB859673. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under accession LAYY00000000. The version described in this paper is version LAYY01000000. One supplementary figure is available with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2015), 65, 3235–3240 DOI 10.1099/ijsem.0.000400 000400 G 2015 IUMS Printed in Great Britain 3235