Khwaja Yunus Ali Uni. J. Vol. 5, Issue 2, December 2022 KYAU Journal, 5(2), 39-51 39 Khwaja Yunus Ali University Journal Publisher homepage: www.kyau.edu.bd OPEN ACCESS ISSN: 2791-3759 (Online), 2521-3121 (Print) Journal homepage:www.journal.kyau.edu.bd Research Article Evaluation of anthelmintic activities and ADME/T properties of Ficus racemosa Linn. Khurshid Jahan Shammi 1 , Safayet Jamil 1 , Asma Akhter 1 , Naznin Shahria 1 , Imran Mahmud 1 * 1 Department of Pharmacy, Khwaja Yunus Ali University, Enayetpur, Sirajgonj-6751, Bangladesh *Correspondence to: imran.mahmud.pharm@kyau.edu.bd (Imran Mahmud, Assistant Professor, Department of Pharmacy, Khwaja Yunus Ali University, Enayetpur, Sirajgonj-6751, Bangladesh) Abstract The aim of this study was to determine the anthelmintic activity of ethanolic extract of F. racemosa against bovine parasite. After that, the isolated compounds were put through molecular docking and an ADME/T evaluation. The current investigation includes the use of ethanol extraction on dried leaves. The anthelmintic activity of the compound was determined with the help of an experimental model utilizing the worm Paramphistomum cervi, Schrödinger-Maestro v10.1 docking fitness was then used to conduct a molecular docking analysis to discover compounds with the highest activity against the Tubulin-Colchicine enzyme. Moreover, the Swiss ADME online-based application was used to review the ADME/T profiles. Preliminary phytochemical screening of ELFR revealed that it contains alkaloids, glycoside, flavonoids, tannins, gums, carbohydrate and quinone. ELFR showed a dose-dependent and statistically significant anthelmintic activity on experimental worm (Paramphistomum cervi). ELFR having a concentration of 200 mg/ml showed lowest paralysis and death time of 8.33 and 12.84 min, respectively, which is comparable with that of standard drug albendazole. Moreover, the molecular docking study reveals that among the seven compounds isolated from the plant, β-sitosterol has the best docking score of -15.4 kcal/mol against Tubulin-Colchicine and ADME/T analysis using web-based tool ensures that the compound has not violated Lipinski’s rule of five indicating its safety consumption. The findings from both in vitro and in silico studies corroborate the anthelmintic activity of Ficus racemosa leaves. The data support beta-sitosterol to be a potential anthelmintic agent worthy of further comprehensive clinical studies and exploring its drug-like properties. Keywords: F. racemosa, Anthelmintic, Molecular docking analysis, ADME/T property 1. Introduction Medicine is the wonder of the world and blessings for mankind. From the very ancient time people used various plant parts as therapeutic aid for alleviating ailments .It is common knowledge that herbs have a wealth of medicinal compounds that can treat a variety of conditions. On the other hand, the ethnopharmacological applications of these medicinal plants may differ depending on the tenets of a certain individual's traditional medical system and the compositions that are made by regional ethnic communities (Alongi et al., 2001).