82 Research Article The Effect of Royal Jelly Administration on the Number of Follicles in Mice (Mus Musculus) Exposed to Noise Florentina Lintang Sekar Arum 1 , Widjiati Widjiati 1 , Wiwiek Tyasningsih 1 , Erma Safitri1, Suryo Kuncorojakti 1 , Budiarto Budiart o1 , Viski Fitri Hendrawan 2 , and Epy Muhammad Luqman 1 * 1 Department of Veterinary Science, Faculty of Veterinary Medicine, University of Airlangga, Kampus C Unair Jl. Mulyorejo Surabaya, Indonesia 2 Department of Reproduction Faculty of Veterinary Medicine Universitas Brawijaya Malang, 65151, Indonesia *Corresponding Author Epy Muhammad Luqman Article History Received: 09.01.2023 Accepted: 21.01.2023 Published: 07.02.2023 Citations: Epy Muhammad Luqman1 et al., (2023); The Effect of Royal Jelly Administration on the Number of Follicles in Mice (Mus Musculus) Exposed to Noise. Hmlyn J Agr; 4(1), 82-85 Abstract: This study was aims to prove that royal jelly can affect fertility in terms of maintaining the number of follicles in the folliculogenesis process of mice that are stressed due to noise exposure. This study used 25 female mice aged 2-3 months with a body weight of 20g. Mice were given royal jelly orally for 28 days and then continued with noise exposure of 94dB. Mice were divided into 5 groups consisting of (K-) neither royal jelly nor exposure to noise, group (K+) was not given royal jelly but was given exposure to noise, group was given royal jelly 1.75 mg/days and noise exposure, the group was given royal jelly 3.50 mg/day and noise exposure, and the group was given royal jelly 5.25 mg/day and noise exposure. The results showed that there was no significant difference in the primary and secondary follicles (p>0.05). However, there was a significant difference in tertiary follicles and de Graff follicles between the normal control group without exposure and the group without royal jelly with exposure, the royal dose group jelly 1.75 mg/day, the royal jelly dose group was 3.50 mg/day, but not significantly different from the royal jelly dose group 5.25 mg/day (p<0.05). It can be concluded that a dose of 5.25 mg/day royal jelly can be effectively used in increasing the number of tertiary and deGraff follicles. Keywords: Folliculogenesis, Mus Musculus, Noise, Reproductive health, Royal Jelly INTRODUCTION Stressed is a condition where the individual's body fails to control the body's balance because sudden environmental changes (Gaol, 2016). Continuous exposure to noise not only results in the organs of hearing, but also has side effects on neurophysiological and psychological (Marliyawati, 2019). Exposure to noise that occurs over a long period of time can induce the assetsof the hypothalamic-pituitary-adrenal (HPA) axis continuously, causing reproductive disorders in folliculogenesis, because there is a disturbance in the secretion of FSH and LH will cause disturbances in folliculogenesis (Stamatiades and Kaiser, 2017). The number of follicles that develop in the ovaries is influenced by the response of follicular granulosa cells to the hormone FSH. Follicles affected by FSH stimuli will grow to the stage of maturation of oocytes (Anwar, 2005). Research conducted by Taixeria et al., (2017) has proven that royal jelly can reduce corticosterone levels in mice induced by cold stress because royal jelly contains 10-HDA fatty acids that can inhibit the production of corticosterone stimulated by ACTH. In addition, royal jelly contains a protein that is predominantly known as Major Royal Jelly Proteins (MJRPs). Royal jelly is currently widely used as a dietary supplement, as it is believed to increase the body's resistance. Therefore, this study aims to see if royal jelly can also prevent the negative impact of exposure to stress due to noise stressors on the number of mice follicles. MATERIALS AND METHODS This research was conducted for two months starting from May to July 2022. This research was conducted in the experimental animal cage of the Faculty of Veterinary Medicine, Universitas Airlangga Surabaya for the maintenance and treatment of experimental animals. The manufacture of histopathological preparations was carried out in the Pathology Division of the Faculty of Animal Medicine, Universitas Airlangga. Examination of histopathological preparations was carried out in the Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga. Animal This study used 25 female mice (Mus musculus) strain Balb/C aged 2-3 month old with an average body weight of 20g were purchased from Pusvetma Surabaya. Mice were acclimatized in the laboratory for 7 days before being placed in a single cage and fed a conventional diet with plenty of water for a total of 12 days of treatment. Material Himalayan Journal of Agriculture Open Access