Contents lists available at ScienceDirect Industrial Crops & Products journal homepage: www.elsevier.com/locate/indcrop Determination of antioxidant enzyme activity and phenolic contents of some species of the Asteraceae family from medicanal plants Adem Güneş a, ⁎ , Şaban Kordali b , Metin Turan c , Ayşe Usanmaz Bozhüyük d a Erciyes University, Faculty of Agriculture, Department of Soil Science and Plant Nutrition, Kayseri, Turkey b Muğla Sıtkı Koçman University, Faculty of Fethiye Agriculture, Department of Plant Protection, Muğla, Turkey c Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, Kayisdagi, Istanbul, Turkey d Iğdır University, Faculty of Agriculture, Department of Plant Protection, Iğdır, Turkey ARTICLE INFO Keywords: Antioxidant Asteraceae Hormone Medicinal plants ABSTRACT Medicnal and aromatic plants are used in different fields depending on the amount of antioxidant and phenolic substances they have. This study was conducted to determine the antioxidant enzyme activity and phenolic contents of some species of the Asteraceae family (Achillea biebersteinii Afan., Achillea millefolium L., Achillea nobilis L., Artemisia absinthium L., Artemisia alba Turra., Artemisia dracunculus L., Artemisia santanicum L., Artemisia vulgaris L., Carthamus tinctorius L., Centaurea cyanus L., Echinacea pallida Nutt., Echinacea purpurea L. Moench., Grindelia robusta Nutt., Helianthus annuus L., Helichrysum plicatum L., İnula helenium L. and Santolina chamaecyparissus L.) for medicines, additive food supplement and cosmetic sector. In, this study, the antioxidant amount of some medicinal and aromatic plants with different properties belonging to Asteraceae family and hormones, phenolic substance and vitamin C and vitamin E were determined. E. purpurea, A. nobilis, G. robusta and S. chamaecyparissus species have more antioxidant, enzymes, hormones and some phenolic substances such as vitamin C and vitamin E, than other species in Asteracae. According to study result, highest amount of gibberellic acid, salicylic acid, indole acetic acid (IAA), abscisic acid (ABA), catalase (CAT) enzyme activity, peroxidase (POD) enzyme activity, superoksid dismutase (SOD) enzyme activity, ascorbate peroxidase (AxPOD) enzyme activity, malondialdehyte (MDA), hydrogen peroxide, total antioxidant, total phenolic, total carotenoid amount, vitamin E contents, vitamin C contents, chlorogenic acid contents, caffeic acids, chloric acid and proantocyanidin were determined in the A. millefolium (819.46 ng μl -1 ), A. dracunculus (91.29 ng μl -1 ), S. cy- parissus L. (1985.08 enzyme unit (EU) g leaf -1 ), S. chamaecyparissus (415.22 EU g leaf -1 ) E. purpurea (83.78 EU g leaf -1 ), A. nobilis (41.91 EU g leaf -1 ), S. chamaecyparissus (140.85 nmol g -1 ), E. purpurea (10.49 μmol g -1 ) A. nobilis (1464 μmol Trolox Equivalents (TE) per g -1 ), E. purpurea (13.34 mg gallic acid (GA) per g -1 fresh weight (FW)), E. purpurea (29.41 g vitamine A 100 -1 ), G. robusta (33.63 g-Alfa tocopherol eg 100 g -1 ), A. nobilis (20.70 g 100g -1 ), E. purpurea (13.51 mg g -1 fw), A. nobilis and S. chamaecyparissus (1.83 mg g -1 fw), S. cha- maecyparissus (143.84 mg g -1 fw) and A. nobilis (83.63%) species, respectively. According to the results ob- tained, it is determined that there are significant changes in the contents of the medicinal and aromatic plants depending on the region and climatic conditions. 1. Introduction Certain herbs and spices have been used not only as spices, but also as medicines and preservatives since ancient times (Dorman et al., 2004). In some new diet programs, some medicinal aromatics are often used to provide physiological benefits and prevent chronic disease (Fares et al., 2011). Depending on the stress conditions, reactive oxygen species (ROS) and other free radicals occur in cells. In such cases, prevention of ROS production or reduction of its effects is crucial for preventing diseases (Tohma and Gulcin, 2010; Wu et al., 2011; Goncalves et al., 2013). Reactive oxygen species and free radicals such as hydrogen peroxide, hypochlorous acid, hydroxyl radicals, and su- peroxide anions are produced by cells depending on cellular functions. However, rapid generation of free radicals causes oxidative damage of biomolecules. This situation causes disorders such as asthma, in- flammatory disease, and cancer (Ghimire et al., 2011). Antioxidants such as catalase-peroxidase provide protection from damage caused by ROS, lipid peroxidation, and protein damage (Valko et al., 2007; Patil https://doi.org/10.1016/j.indcrop.2019.05.042 Received 31 December 2018; Received in revised form 13 May 2019; Accepted 14 May 2019 ⁎ Corresponding author. E-mail address: ademgunes@erciyes.edu.tr (A. Güneş). Industrial Crops & Products 137 (2019) 208–213 0926-6690/ © 2019 Elsevier B.V. All rights reserved. T