This study is derived from the doctoral thesis named “Examination of the relationship betwee n aerobic endurance and mental toughness in professional football players” accepted at Aydın Adnan Menders University Health Sciences Institute. International Journal of Turkish Sport and Exercise Psychology Research article Physiological and Mental Toughness in Football: An Application on Professional Footballers Deniz Ünver 1 , Kürşat Karacabey 2 1 Çanakkale Onsekiz Mart University, Health Sciences Faculty, Çanakkale 2 Aydın Adnan Mneders University, Sport Sciences Faculty, Aydın Journal homepage: http://dergipark.org.tr/tr/pub/ijtsep Uluslararası Türk Spor ve Egzersiz Psikolojisi Dergisi Article history: Received 13 September 2022 Received in revised form: December 2022 Accepted: December 2022 Available online: December 2022 Doi: https://doi.org/10.55376ijtsep.1226091 In this research, which was carried out to determine and reveal the physiological and mental endurance levels of professional football players; Yo-Yo test level-1 and mental toughness scale were used. The universe and sample of the study were selected in the Turkish football federation professional leagues, super league (4 teams, n=55), first league (4 teams, n=94), second league (6 teams, n=94) and third leagues between the years 2019-2020. (5 teams, n=102) consists of 391 professional Turkish football players. Within the scope of the research, the data obtained from professional Turkish football players were analyzed with the SPSS 22.0 package. As a result of this research, which examined the aerobic and mental endurance of professional football players; It has been determined that the mental endurance levels of married football players are higher than the single football players, the mental endurance of the central midfielders is slightly higher than the other players, and as the physical endurance levels of the football players increase, their mental endurance increases accordingly. Keywords Yo-Yo, MaxVO2, Psychology, Statistics, Midfielder, Success ABSTRACT ARTICLE INFO