Supporting information for Non-Ionic Amphiphilic Homopolymers: Synthesis, Solution Properties and Biochemical Validation K. Shivaji Sharma a,1 , Grégory Durand a,b,* , Frank Gabel c, d, e , Paola Bazzacco f , Christel Le Bon f , Emmanuelle Billon-Denis f , Laurent J. Catoire f , Jean-Luc Popot f,* , Christine Ebel c, d, e * and Bernard Pucci a,b a Université d'Avignon et des Pays de Vaucluse, Equipe Chimie Bioorganique et Systèmes Amphiphiles, 33 rue Louis Pasteur, F-84000 Avignon, France; b Unité Mixte de Recherche 5247, Centre National de la Recherche Scientifique and Universités de Montpellier 1&2, Faculté de Pharmacie, Institut des Biomolécules Max Mousseron, 15 avenue Charles Flahault, F-34093 Montpellier Cedex 05, France; c CEA, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, F-38027, Grenoble, France d CNRS, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France e Université Joseph Fourier – Grenoble 1, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France f Unité Mixte de Recherche 7099, Centre National de la Recherche Scientifique and Université Paris 7, Institut de Biologie Physico-Chimique, 13 rue Pierre-et-Marie Curie, F-75005 Paris, France. 1 Present address: Département de Chimie et Faculté de Pharmacie, Université de Montréal, Pavillon J. A. Bom- bardier, CP 6128 Succursale Centre Ville, Montréal QC H3C 3J7, Canada *Corresponding authors: gregory.durand@univ-avignon.fr ; christine.ebel@ibs.fr ; jean-luc.popot@ibpc.fr Table of contents Pages Materials, general procedures and instrumentation for the synthesis S2 Figure S1 Particle size distribution by volume of NA29 determined by DLS at different concentrations and at different temperatures S2 Figure S2 Ability of NA h Pols NA10, NA29 and NA11 to keep BR soluble in aqueous solution after detergent removal by adsorption onto BioBeads. S3 Figure S3 UV/visible absorbance spectra of BR after trapping with APols NA10, NA29 or A8-35 at a protein/polymer ratio of 1:5 w/w S3 Figure S4 ASEC elution profiles of NA11 particles and OmpX/NA11 complexes. Detection at 220 nm. S4 Figure S5 Sedimentation velocity analysis of dOmpX/NA11 complexes S4 Table S1 Particle size distribution by volume of various NA h Pols at different concentrations determined by dynamic light scattering S5 Table S2 Particle size distribution by volume of various NA h Pols at different temperatures determined by dynamic light scattering S5