Vol.:(0123456789) 1 3
Advances in Traditional Medicine
https://doi.org/10.1007/s13596-020-00466-2
RESEARCH ARTICLE
Baccharis trimera (carqueja) promotes gastroprotection
on ethanol‑induced acute gastric ulcer
Ana Carolina Silveira Rabelo
1,2
· Fernanda Caetano Camini
1
· Melissa Maia Bittencourt
3
· Kíssyla Lacerda
3
·
Wanderson Geraldo de Lima
3
· Daniela Caldeira Costa
1
Received: 10 July 2019 / Accepted: 29 May 2020
© Institute of Korean Medicine, Kyung Hee University 2020
Abstract
One of the factors that can promote gastric ulcer is the acute and/or chronic consumption of ethanol. One of the most used
therapeutic measures is the use of proton pump inhibitor, like omeprazole. However, these drugs have several side efects,
making it necessary to search for new alternatives. To investigate the gastroprotective efect of Baccharis trimera (carqueja)
we used an ethanol-induced acute gastric ulcer model. For this, gastric ulcer was induced in rats using absolute ethanol for
2 consecutive days. After analysis of the stomach, an increase in oxidative stress was observed, as well as the reduction of
the antioxidant defense. In addition, we observed in histology that there was an increase in infammatory cells, necrosis,
hemorrhage and hyperemia. Treatment with B. trimera was able to decrease oxidative damage as well as improve antioxidant
defense. There was also a decrease in infammation, collagen area and hemorrhage. All the benefcial efects promoted by
carqueja were similar to those found by the positive controls used in our study (vitamin C and omeprazole). Analyzing our
data, we can conclude that B. trimera presents a gastroprotective efect and should be further studied for its application in
gastric ulcer therapy.
Graphical abstract
After 2 days of consumption of absolute ethanol, the animals’ stomachs showed an increase in oxidative stress, characterized
by reduction of oxidized glutathione (GSSG) and increase of TBARS and carbonylated protein. This situation led to stomach
infammation, necrosis, hemorrhage and hyperemia. These was minimized by the consumption of Baccharis trimera extracts
(Bt), since it was able to decrease oxidative damage and increasing GSSG, reduced glutathione (GSH) and catalase (CAT).
Ana Carolina Silveira Rabelo, Fernanda Caetano Camini and
Melissa Maia Bittencourt contributed equally to this work.
* Ana Carolina Silveira Rabelo
ana_rabelo@usp.br; ana_far3p@yahoo.com.br
1
Laboratory of Metabolic Biochemistry (LBM), Department
of Biological Sciences (DECBI), Center of Research
in Biological Science (NUPEB), Federal University of Ouro
Preto (UFOP), Ouro Preto 35400-000, Brazil
2
Laboratory of Stem Cell, Department of Anatomy
of Domestic and Wild Animals and Department of Surgery
(VCI), Faculty of Veterinary Medicine and Animal Science,
University of São Paulo (USP), São Paulo 05508 270, Brazil
3
Laboratory of Morphology, Department of Biological
Sciences, Graduate Program in Biological Sciences,
Federal University of Ouro Preto (UFOP), Ouro Preto,
MG 35400-000, Brazil