JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga 2023 January, XIV(01) Effects of Javanese Ginseng Root Extracts (Talinum triangulare W.) on Thickness of Seminiferous Tubules of Male Rats (Rattus norvegicus) Exposed by Cigarette Smoke Rifqi Misbahuddin Nur 1 , Anung Putri Ilahika 2* , Desy Andari 3 1 Faculty of Medicine, University of Muhammadiyah Malang, Malang, Indonesia. 2 Department of Anatomy, Faculty of Medicine, University of Muhammadiyah Malang, Malang, Indonesia. 3 Department of Histology, Faculty of Medicine, University of Muhammadiyah Malang, Malang, Indonesia. A B S T R A C T Introduction: Cigarette smoke contains various radical chemical compounds that can increase oxidative stress, which may cause lipid peroxidation in the body and affect various different organs. This study aimed to examine the effects of Javanese ginseng root extracts (Talinum triangulare W.) on the thickness of seminiferous tubules of white male rats (Rattus norvegicus) exposed to cigarette smoke. Methods: This was an experimental study with a randomized controlled method and a post-test control group design. Post-test control group design with 15 white male rats was divided into five groups: a normal group, a control group (exposed to cigarette smoke), and three treatment groups (exposed to cigarette smoke and Javanese ginseng root extract with doses of 12.5mg, 25mg, and 50mg/200grBW, respectively) for 30 days. The seminiferous tubule thickness was observed using H&E staining under a light microscope with 200x magnification in five fields view. Results: One-way ANOVA showed that Javanese ginseng root extracts significantly affected seminiferous tubule thickness (p = 0.000). With a dose of 12.5mg/200mgBW, Javanese ginseng root extract started to show a significant effect (p = 0.000) on the thickness of the epithelium of the seminiferous tubules. The group exposed to cigarette smoke had an average thickness of seminiferous tubules of 7.92 µm, the normal group had an average thickness of 9.13 µm, and treatment groups with doses 12.5mg, 25mg, and 50mg/200gBW had an average thickness of 11.85 µm, 12.7µm, and 14.69 µm, respectively. Conclusion: Javanese ginseng root extract increased seminiferous tubule thickness in white male rats exposed to cigarette smoke. A R T I C L E I N F O Article history: Received 27-07-2022 Received in revised form 26-12-2022 Accepted 4-12-2023 Available online 10-01-2023 Keywords: Cigarette smoke, Javanese ginseng root, Seminiferous tubules, Tobacco use. Cite this as: Nur RMN, Ilahika AP, Andari D. Effects of Javanese Ginseng Root Extracts (Talinum triangulare W.) on Thickness of Seminiferous Tubules of Male Rats (Rattus norvegicus) Exposed by Cigarette Smoke. JUXTA J Ilm Mhs Kedokt Univ Airlangga 2023; 14: 6–11. * Correspondence: putri@umm.ac.id JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga p-ISSN: 1907-3623; e-ISSN: 2684-9453 DOI: 10.20473/juxta.V14I12023.6-11 Open access under Creative Commons Attribution-ShareAlike 4.0 International License (CC-BY-SA) ORIGINAL ARTICLE ORIGINAL ARTICLE