_____________________________________________________________________________________________________ *Corresponding author: E-mail: jeromeihuma@gmail.com; Asian Journal of Biology 15(3): 24-31, 2022; Article no.AJOB.90978 ISSN: 2456-7124 Antimicrobial Activities of the Leaf Extracts of Ficus sycomorus Linn. on Helicobacter pylori and Citrobacter freundii J. O. Ihuma a* , A. Z. Koggie a , T. I. Famojuro b , L. O. Ugboji a and T. D. Malgwi c a Department of Biological Sciences, Bingham University, P.M.B. 005, Karu, Nasarawa State, Nigeria. b Department of Pharmacognosy, Bingham University, P.M.B. 005, Karu, Nasarawa State, Nigeria. c Department of Community Medicine, Nnamdi Azikiwe University, Anambra State, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Author AZK designed the methodology and supervised the study. Authors JOI and LOU managed the analysis of the study and the literature of the study. Authors JOI and TDM prepared the first draft of the manuscript. Author TIF revised the manuscript. All authors read and approved the final manuscript. Article Information DOI: 10.9734/AJOB/2022/v15i330240 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/90978 Received 18 June 2022 Accepted 23 August 2022 Published 25 August 2022 ABSTRACT Aims: The aim of this study is to determine the antimicrobial activities of Ficus sycomorus leave extract on selected bacteria. Phytochemical screening showed the presence of tannins, saponins, alkaloids and flavonoids, it also revealed the absence of glycoside. Place and Duration of Study: Microbiology laboratory, Biological Sciences Department, Bingham University Karu, Nasarawa State, Nigeria. Methodology: Plant extracts were prepared by boiling, soaking and methanol extraction. 96-well plates dilution method for determination of Minimum Inhibition Concentraction (MIC) and Minimum Bactericidal Concentration (MBC) were carried out. Results: The MBC for Helicobacter pylori and Citrobacter freundii showed antimicrobial activities at 400 μl each. The plant has exhibited strong antimicrobial activity on Helicobacter pylori and Citrobacter freundii. Conclusion: The antimicrobial compounds produced by plant extracts are active against bacteria. It is expected that plant extracts showing target sites other than those used by antibiotics will be active against drug-resistant microorganisms. Original Research Article