hands at later stages. How DSPN affects the somatosensory nerve function in the hands is understudied. Aim: In this study, we aimed to (1) establish small and large diameter nerve fibre dysfunction in hands in people with DSPN in hands, and (2) assess whether people with diabetes without DSPN and people with DSPN in their feet but without neuropathy symptoms in their hands already show signs of small and large diameter nerve fibre dysfunction. Method: A total of 125 people with and without diabetes were involved in this cross-sectional study. We used nerve conduction tests and corneal confocal microscopy for objective assessments of DSPN. Approximately 30 participants met the selection criteria to be allocated to one of the following four study groups: healthy participants, people with diabetes without DSPN, people with DSPN in feet but not hands; and people with DSPN in hands and feet. We assessed hot /cold and mechanical detection and pain thresholds in the hands using a standardised Quantitative Sensory Testing (QST) protocol. One-way between-group analyses of variance were performed to compare groups. The level of sig- nificance was set at p<0.05. Results: People with DSPN in hands and feet had the most sev- ere loss of thermal and mechanical sensationin their hands com- pared to the other groups. Interestingly, people with DSPN in only their feet already presented with a loss of nerve function in hands without reporting any symptoms. People with diabetes without DSPN also already showed signs of nerve fibre dysfunction, but this was less pronounced than for people with DSPN in only their feet. Hand function and quality of life were poorer in people with DSPN in hands and feet compared to the other groups. Discussion: People with diabetes present with a somatosen- sory profile in their hands characterised by a loss of function. Interestingly, the pattern of widespread loss of small and large diameter nerve fibre function is comparable to feet. This loss of function was more severe in people with DSPN in hands and feet as reflected in poorer quality of life and hand function. Early changes could be detected in people with DSPN in feet only and in people with diabetes without DSPN. If these signs are early indicators of a progressive development of DSPN in hands, we might see many more nerve-related hand complications in the future in people with diabetes. Diabetes Research and Clinical Practice 186S (2022) 109683 https://doi.org/10.1016/j.diabres.2022.109683 IDF21-0535 Relationship between diabetic polyneuropathy and brain derived neurotrophic factor in newly diagnosed type 2 diabetes O. Stepura a , N. Zherdova a , b , T. Drevytska c , B. Mankovsky a,b a State Scientific Institution ‘‘Center for Innovative Medical Technologies of the National Academy of Sciences of Ukraine”, Department of diagnosis and treatment of metabolic diseases, Kyiv, Ukraine b P.L. Shupyk National Medical Academy of Postgraduate Education, Department of Diabetology, Kyiv, Ukraine c Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Department of hypoxic states investigation, Kyiv, Ukraine Background: BDNF is a protein, which is encoded by the BDNF gene, of the class of neurotrophins, which stimulates growth, dif- ferentiation, development, survival and renewal of neurons and synapses. Diabetic polyneuropathy (DN) is most common compli- cation in diabetes mellitus (DM). Aim: The aim of this study was to investigate relationship between level of BDNF gene and diabetic polyneuropathy in newly diagnosed type 2 DM. Method: We examined 52 patients with newly diagnosed type 2 DM, 16 males and 36 females (aged 60,9±1,55 years, HbA1c – 7,77 ±0,18 %) (data are presented everywhere as mean±SEM). All patients were diagnosed for the presence of diabetic polyneu- ropathy by Toronto scale. Real-time PCR analysis was performed for quantitative evaluation of BDNF gene. Results presents as B (95% CI). The statistical analysis was performed using SPSS-25. Results: Mild DN was diagnosed in 28,8% patients, moderate DN - 19,2% and severe DN in 19,2 % patients with newly diagnosed type 2 DM. We found negative correlation between the total score of Toronto scale and levels of BDNF gene, B (95 % CI) -28,5 (-55,79/- 1,21), p=0,01. Discussion: DN is a common complication on newly DM type 2 and BDNF gene are some role for its development revealed in our study. Diabetes Research and Clinical Practice 186S (2022) 109684 https://doi.org/10.1016/j.diabres.2022.109684 IDF21-0568 Peripheral Neuropathy and potential risk factors in patients with Diabetes Mellitus in Kano, Northwestern Nigeria R. Liyu a , A.E. Uloko a , I.D. Gezawa a a Aminu Kano Teaching Hospital, Internal Medicine, Kano, Nigeria Background: Peripheral Neuropathy is a common complica- tion of DM and a cardinal event in the pathway to foot ulceration in many patients. It may be preventable if detected early and promptly managed. Aim: To determine the prevalence of peripheral neuropathy and its potential risk factors in patients with DM attending two hospitals in Kano, Nigeria. Method: In a cross – sectional descriptive study consenting patients with DM who fulfilled the inclusion criteria were recruited from the diabetes outpatient clinic and wards of two hospitals in Kano. Questionnaires were used to obtain Socio- demographic data and clinical characteristics of the study sub- jects.Vibration perception threshold using biothesiometer was used to assess for presence of peripheral neuropathy. Results: We recruited 394 patients with DM (163 males and 231 females) with mean age and duration of DM of 50.8±12.5years and 7.72±6.65years respectively. Ninety five percent of the study sub- jects had type 2DM. 149(37.2%) of the study subjects had VPT > 25 volts which is significant for PN. Older age, level of education, longer duration of DM, HTN, PVD, proteinuria, diabetic retinopa- thy, presence of DFU and foot deformities were significant predic- tors of PN. The independent risk factors for PN after multivariate regression were older age, longer duration of DM, proteinuria, presence of foot deformities and DFU. Discussion: Peripheral neuropathy still remains one of the most complications of DM which leads to development of DFU and subsequently amputation. Screening with early identification, education and treatment of associated risk factors may go a long way in reducing one of the cardinal event to foot ulceration. 10 diabetes research and clinical practice 186S (2022) 109667