Volume Issue 1 BKAP-529 [1-4] REVIEW ARTICLE Bhartiya Krishi Anusandhan Patrika Micropropagation of Strawberry Crop ( Fragaria ananassa): A Review Akash Sanjay Valliath 1 , Radhajogita Mondal ABSTRACT The objective of this review paper is to summarize on the history, advantages, disadvantages of in vitro micropropagation of strawberry crop. Since the traditional method of propagation, that is, the use of runners as propagating material, is not that efficient because it leads to the spread of viral diseases. We have to go for in vitro micropropagation, the crops also yield more when compared to traditional methods of propagation. The paper also reviews a study done to develop a protocol for large-scale propagation of strawberry cv. ‘Sweet Charlie’ and ‘Winter Dawn’ using the micropropagation technique. Shoot cultures were obtained from shoot tips on Murashige and Skoog (MS) medium with 4% table sugar, 0.75% agar, 5 mg L -1 6-benzyladenine and 0.01 mg L -1 kinetin. These shoots were multiplied and maintained on MS medium with 1 mg L -1 6-benzyladenine and 0.1 mg L -1 kinetin. Rooting of in vitro raised shoots was successfully conducted by pulse treatment with 500 mg L -1 indole-3-butyric acid for 30 s and subsequent culturing in MS medium with 1 mg L -1 indole-3-butyric acid, 0.1 g L -1 activated charcoal and 6% table sugar. Plants were successfully acclimatized and survived in field conditions. Key words: Fragaria, Micropropagation, Plant propagation, Strawberry. Department of Horticulture, Lovely Professional University, Phagwara, Jalandhar-144 402, Punjab, India. Corresponding Author: Akash Sanjay Valliath, Department of Horticulture, Lovely Professional University, Phagwara, Jalandhar- 144 402, Punjab, India. Email: akashvalliath@gmail.com How to cite this article: Valliath, A.S. and Mondal, R. (2023). Micropropagation of Strawberry Crop ( Fragaria ananassa): A Review. Bhartiya Krishi Anusandhan Patrika. Submitted: 09-05-2022 Accepted: 24-01-2023 Online: Strawberry (Fragaria ananassa Duch.), belonging to the family Rosaceae, is one of the important small fruits. It is produced over an area of 1800600 hectares spread over 71 countries. The annual world production of 9,125,913 tonnes (FAOSTAT, 2017). All the strawberry cultivars present today all octoploid (2n=56) and well adapted to a wide range of climatic conditions. Strawberry is commercially grown in Jammu and Kashmir, Himachal Pradesh, Uttar Pradesh, Maharashtra, West Bengal, the Nilgiri Hills and sections of Delhi, Haryana and Punjab in India. Strawberries have always been a popular delicacy due to its flavour, taste, freshness, freezing and processing capabilities. It contains relatively high quantities of ellagic acid, which has a wide range of biological activity. Strawberries contain many important dietary components including vitamins, minerals, folate and fibre and are a rich source of phytochemical compounds mostly represented by polyphenols (Giampieri et al., 2012). Runners are used to propagate the strawberry crop and viral infections are frequently transmitted through runners. As a result, in-vitro micropropagation techniques have been proved to be a viable alternative to traditional runner production for mass propagation (Singh, 2002) of virus free planting material (Lal et al., 2003; Kanwar et al., 2013). It’s also a known fact that tissue cultured strawberry plants have higher yield as compared to conventionally grown plants (Cameron and Hancock, 1986). Despite the benefits of micropropagation, the expensive cost of plant production has prevented this approach from being used in the past. Certain elements, however, can be tweaked to make micropropagation more cost-effective. Micropropagation in strawberry Micropropagation or tissue culture is the process of rapidly multiplying plant stock material to produce many progeny plants, using plant tissue culture methods. The first report of in vitro strawberry propagation by Boxus (1974), there have been many reports on types of medium, plant growth regulators, genotypes and types of explants used in strawberry regeneration. Nevertheless, there are still problems associated with the regeneration of strawberry explant, i.e., meristem; for example, the highest percentage of explant producing shoots for cv. Elsanta was only 4% (Debnath, 2006), which seems to be insufficient for commercialization (Boxus, 1974). Young strawberry plantlets, obtained from meristems, are initially maintained in a medium containing undiluted Knop solution, the micro-elements used by Murashige and Skoog (1962), nicotinic acid 0.5 mg/L, pyridoxine HCl 0.5 mg/L, glycine 2.0 mg/L, thiamine HCl 0.1 mg/L, meso-inositol 100.0 mg/L, indolybutyric acid 1.0 mg/L, glucose 40.0 g/L,