TYPE Original Research PUBLISHED 11 January 2023 DOI 10.3389/fmicb.2022.957830 OPEN ACCESS EDITED BY Nejat Duzgunes, University of the Pacific, United States REVIEWED BY Liu Sidang, Shandong Agricultural University, China Mira C. Patel, Centers for Disease Control and Prevention, United States *CORRESPONDENCE Heiko Adler h.adler@helmholtz-muenchen.de Claudia A. Staab-Weijnitz staab-weijnitz@helmholtz- muenchen.de SPECIALTY SECTION This article was submitted to Virology, a section of the journal Frontiers in Microbiology RECEIVED 31 May 2022 ACCEPTED 19 December 2022 PUBLISHED 11 January 2023 CITATION Nakayama M, Marchi H, Dmitrieva AM, Chakraborty A, Merl-Pham J, Hennen E, Le Gleut R, Ruppert C, Guenther A, Kahnert K, Behr J, Hilgendorff A, Hauck SM, Adler H and Staab-Weijnitz CA (2023) Quantitative proteomics of differentiated primary bronchial epithelial cells from chronic obstructive pulmonary disease and control identifies potential novel host factors post-influenza A virus infection. Front. Microbiol. 13:957830. doi: 10.3389/fmicb.2022.957830 COPYRIGHT © 2023 Nakayama, Marchi, Dmitrieva, Chakraborty, Merl-Pham, Hennen, Le Gleut, Ruppert, Guenther, Kahnert, Behr, Hilgendorff, Hauck, Adler and Staab-Weijnitz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Quantitative proteomics of differentiated primary bronchial epithelial cells from chronic obstructive pulmonary disease and control identifies potential novel host factors post-influenza A virus infection Misako Nakayama 1,2 , Hannah Marchi 3,4 , Anna M. Dmitrieva 5 , Ashesh Chakraborty 1 , Juliane Merl-Pham 6 , Elisabeth Hennen 1 , Ronan Le Gleut 3 , Clemens Ruppert 7 , Andreas Guenther 7 , Kathrin Kahnert 8 , Jürgen Behr 8 , Anne Hilgendorff 1 , Stefanie M. Hauck 6 , Heiko Adler 5,9 * and Claudia A. Staab-Weijnitz 1 * 1 Institute of Lung Health and Immunity and Comprehensive Pneumology Center with the CPC-M BioArchive, Helmholtz Zentrum München, Member of the German Center of Lung Research (DZL), Munich, Germany, 2 Division of Pathogenesis and Disease Regulation, Department of Pathology, Shiga University of Medical Science, Otsu, Japan, 3 Core Facility Statistical Consulting, Helmholtz Zentrum München, Munich, Germany, 4 Faculty of Business Administration and Economics, Bielefeld University, Bielefeld, Germany, 5 Research Unit Lung Repair and Regeneration, Helmholtz Zentrum München, Member of the German Center of Lung Research (DZL), Munich, Germany, 6 Metabolomics and Proteomics Core, Helmholtz Zentrum München, Neuherberg, Germany, 7 Department of Internal Medicine, Medizinische Klinik II, Member of the German Center of Lung Research (DZL), Giessen, Germany, 8 Department of Medicine V, Ludwig Maximilian University (LMU) Munich, Member of the German Center of Lung Research, University Hospital, Munich, Germany, 9 Institute of Asthma and Allergy Prevention, Helmholtz Zentrum München, Member of the German Center of Lung Research (DZL), Munich, Germany Background: Chronic obstructive pulmonary disease (COPD) collectively refers to chronic and progressive lung diseases that cause irreversible limitations in airflow. Patients with COPD are at high risk for severe respiratory symptoms upon influenza virus infection. Airway epithelial cells provide the first-line antiviral defense, but whether or not their susceptibility and response to influenza virus infection changes in COPD have not been elucidated. Therefore, this study aimed to compare the susceptibility of COPD- and control-derived airway epithelium to the influenza virus and assess protein changes during influenza virus infection by quantitative proteomics. Materials and methods: The presence of human- and avian-type influenza A virus receptor was assessed in control and COPD lung sections as well as in fully differentiated primary human bronchial epithelial cells (phBECs) by lectin- or antibody-based histochemical staining. PhBECs were from COPD lungs, including cells from moderate- and severe-stage diseases, and from age-, Frontiers in Microbiology 01 frontiersin.org