International Journal of Science and Research (IJSR) ISSN: 2319-7064 Impact Factor (2018): 7.426 Volume 8 Issue 2, February 2019 www.ijsr.net Licensed Under Creative Commons Attribution CC BY Contamination of Raw Cow Milk by Residues of Organochlorine Pesticides and Veterinary Products: Case of Daloa (Côte d'Ivoire) Ano Guy Serge EHOUMAN 1 , Djédoux Maxime ANGAMAN 2 , Kouassi Narcisse ABOUA 3 1, 2 Université Jean Lorougnon GUEDE Daloa, Département Biochimie-Microbiologie, Laboratoire Agrovalorisation, BP 150 Daloa (Côte D’Ivoire) 3 Université NANGUI Abrogoua Abidjan, UFR des Sciences et Gestion de l’Environnement (SGE), Laboratoire des Sciences de l’Environnement (LSE), 02 BP 801 Abidjan 02 Abstract: This study was undertaken to examine the dietary risks that can result from human exposure to organochlorine pesticide residues and veterinary products through the consumption of fresh cow's milk. The various chromatographic analyzes carried out on the various samples taken revealed a contamination of this foodstuff rather popular with the population of Daloa. Quantified levels range from 0.004 to 0.048 mg.kg-1 per organochlorine pesticide residue with an overall mean loading of 0.083 mg.kg-1. At the level of veterinary products, the result indicates average concentrations ranging from 1.085 to 3.125 μg.kg -1 with an overall average load of 1.698μg.kg-1. Keywords: Fresh cow milk, organochlorine pesticide residues, residues of veterinary products, Daloa (Côte d'Ivoire) 1. Introduction Milk is a food of high nutritional value; it represents a nutritional complex necessary for the needs of the organism [1]. But today the consumer does not expect only a food that contains an untold number of nutrients but rather that it is good for health [2]. Faced with this situation, food security has become in recent years the main concern of the agri-food industry and health professions. [3]. It has become the leitmotif of the media, politics, societies and even families. Foods that are consumed should meet quality criteria for diets that support good nutritional and health status [4], [5]. Unfortunately, large-scale nutritional problems, whether persistent or emerging, do not spare any continent despite the efforts made. Among this panoply of difficulties facing the quality of the foods we consume, the presence of chemicals in our food is an important factor. It is even shown through various studies that the average diet is composed of many chemicals [6], [7]. Chemicals (pesticides, veterinary products) used for the protection of plants, animals and food and against pests and diseases are found in the form of residues in our food [8]; Thus, foods formerly considered as a source of energy are transformed over the years into a vector of toxic. This sad fact worries consumers today. Milk and dairy products are perishable foods. High qualitystandards must be maintained across all production lines to maintain consumer confidence. This trust is essential to promote the consumption of dairy products. The milk that leaves the farm must be of irreproachable quality. The aim of this study is to evaluate the level of contamination of raw cow's milk, a high-consumption food in Daloa (west-central Côte d'Ivoire) with organochlorine pesticide residues and veterinary products. 2. Material and Methods 2.1. Sampling of the biological material The biological material that is the subject of this study is fresh cow's milk from “Abattoir 2”, district of the city of Daloa (west-central Côte d'Ivoire). This district is presented as an area for cattle breeding and cow's milk production. Milk sampling was conducted at ten different breeders. 2 L of milk were collected from each farmer. (1L for the detection of organochlorine pesticide residues and 1L for the determination of veterinary products). A total of 20 L of milk was used for analysis. The milk collected is put into sterile glasses, covered with aluminum foil and sealed to limit the oxidation phenomena. The samples were conveyed the same day of the sampling in a cooler containing ice in Abidjan at the National Laboratory for Agricultural Development Support (LANADA) where the analyzes were carried out the next day. a)Collection of the milk b)conservation of the milk collected Figure 1: Collection and conservation of cow's milk 2.2. Chromatographic analysis of pesticide residues and veterinary products The location of organochloric pesticides residues and veterinary products is made through residual method in order to determine all the residues of these products [9]. These different residues were measured from cow’s milk fat. To extract these residues, the samples were deposited at Paper ID: ART20195491 10.21275/ART20195491 1631