Int. J. Pharm. Investigation, 2022; 12(3) : 386-390 386 International Journal of Pharmaceutical Investigation, Vol 12, Issue 3, Jul-Sep, 2022 Original Article INTRODUCTION Te primary goal of ocular medicine administration is to get better the already accessible conventional dosage shape as well as to make use of innovative drug delivery systems to improve therapeutic efcacy. Te use of eye drops as a topical treatment for people with eye problems is extremely prevalent, because of continual lachrymal secretion, tear turnover, blinking, refex, moreover quick nasolachrymal drainage, traditional eye drops have a low ocular bioavailability. Usual drainage of an administered dosage begins almost instantly afer administration furthermore is fnished in less than 5 min. 1 As a decision, a regular infux of concerted mixture ensures required to obtain these expected healing efects. To address these issues, researchers have looked into a variety of ocular formulations, including viscous solutions, ointments, gels, nanoparticles, and polymeric inserts. 2 Tese vehicles occupy enhanced corneal contact time to varying degrees, but they have not been universally adopted due to obscured vision or a lack of patient compliance. As a decision, achieving adequate ocular bioavailability afer topical distribution of a medicine to the eye is a difculty that has yet to be overcome. 3 Timolol maleate (TM) was used as the model medication in this investigation. Te most ofen used medicine in the treatment of glaucoma is TM, which is a non-selective beta-blocker. Glaucoma is the world’s next to the mass routine source of darkness. It afects around 70 million people all over the world. 4 TM was chosen above other medications in the same class because it had a faster onset, better tolerance, and fewer adverse efects. Tis medication reduces intraocular pressure by inhibiting the generation of aqueous humor in the ciliary epithelium by blocking sympathetic nerve terminals. Te goal of this work was to fnd a way for dispersing proniosomal gel including TM within the in-situ gel to improve the mucoadhesive and long-term release behaviour of the gel. MATERIALS AND METHODS Materials Yarrow chem products, Mumbai, India, provided Timolol maleate, Lecithin, and Poloxamer. Loba Chemie Pvt. Ltd, Mumbai, India, provided the cholesterol. Rolex Chemical Industries in Mumbai, India, provided Span 60. Spectrum Chemicals in Mumbai, India, provided Brij 72. S D Fine Chemicals in Mumbai, India, provided Tween 80. Each chemical utilised were of the highest quality. Methods pre-formulation studies determination of solubility of timolol maleate By using the shake fask method, the solubility of Timolol maleate in aqua, ethyl alcohol, PBS pH 6.8, PBS pH 7.4, and ether was measured. In a fask containing 10 ml of each solvent, an excess amount of the medication was introduced. Te mixtures were then agitated at 100 rpm for 24 hours inside a thermostatically managed water bath at 370.5°C, fltered, diluted, and spectrophotometrically assessed at 294.4 nm using a UV spectrophotometer against a blank that had been treated similarly. 5 Standard curve for timolol maleate Te medication (10 mg) was precisely weighed and put within a 100 mL measuring fask. To make this stock solution-I, the drug was dissolved in 7.4 pH bufer solution and diluted until 10 mL with the similar Formulation and Evaluation of Timolol Maleate Proniosomal Gel for Ocular Drug Delivery Jayatheertha S Lokapur*, Prakash S Goudanavar, Arpitha J Lokapur, Ankit Acharya, Sandip A Murtale Department of Pharmaceutics, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B. G. Nagara, Nagamangala, Mandya, Karnataka, INDIA. ABSTRACT Introduction: The objective of this task is to succeed with the trial of an ocularly ef fcient Timolol maleate formulation produced from prolonged proniosomal gel niosomes to get signifcant therapy for glaucoma. Methods: Cholesterol, Lecithin, Span 60, Brij 72, and Tween 80 in various concentrations were used to create Timolol maleate loaded proniosomal gel derived niosomes utilizing the phase coacervation method. Found on these outcomes of entrapment ef fciency and in-vitro release, an optimised batch of proniosomal gel-produced niosomes was chosen. By spreading proniosomes in an in-situ gelling method, timolol maleate proniosomal gel-derived niosomes were created. Fourier transforms infrared spectroscopy (FTIR) experiments confrmed each interaction study. Wetting agent with additive effects on entrapment ef fciency along with in-vitro drug release manner based on proniosomal gel-produced niosomes was investigated. Cholesterol, Lecithin, Span 60, Brij 72, and Tween 80 in various quantities were used in a coacervation technique, by dispersing proniosomes in an in-situ gelling method, a timolol maleate proniosomal gel was created. Results: The FTIR analyses revealed no signs of interaction between the medicine and the excipients, indicating that they are compatible. At the end of 12 hr, formulations T2 and T10 had 99.98 percent and 99.90 percent drug release, respectively. Conclusion: Starting with these fndings secured, it can be closed this one proniosomal gel derived niosomes might be a satisfactory alternative to conventional eye drops as they exhibited elevated penetrability with sustained-release actions. Keywords: Timolol maleate, Proniosomal gel, Phase co-acervation technique, Ocular delivery, Niosomes. Correspondence Mr. Jayatheertha S Lokapur Ph.D. Research Scholar, Department of Pharmaceutics, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B. G. Nagara-571448, Nagamangala, Mandya, Karnataka, INDIA. Email id: jayatheertha.lokapur@gmail.com DOI: 10.5530/ijpi.2022.3.65 Copyright © 2022 Author(s) et al. Exclusive Licensee Phcog.Net. Distributed under a Creative Commons Attribution License (CC BY 4.0). This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.