Journal of Medicine, Physiology and Biophysics ISSN 2422-8427 An International Peer-reviewed Journal Vol. 13 2015 1 Studying the Virucidal and Biocidal Effects of Electrochemically Activited Anolyte and Catholyte Types of Water on Classical Swine Fever Virus (CSF) and Bactrerium E. coli DH5 Georgi Gluhchev 1* Ignat Ignatov 2 Stoil Karadzhov 3 Georgi Miloshev 4 Nikolay Ivanov 5 Oleg Mosin 6 1. PhD, Assoc. Professor; Institute of Information and Communication Technologies, Bulgarian Academy of Science (BAS) Acad. G. Bonchev Street, bl. 2, Sofia 1113, Bulgaria 2. DSc, Professor, Scientific Research Center of Medical Biophysics (SRCMB), N. Kopernik Street, 32, Sofia 1111, Bulgaria 3. DSc., Professor, Bulgarian Association of Activated Water, Kutuzov blvd, 39, 1619 Sofia, Bulgaria 4. PhD, Assoc. Professor, Institute of Molecular Biology, Bulgarian Academy of Science (BAS), Acad. G. Bonchev Street, bl. 21, Sofia 1113, Bulgaria 5. Dipl. Eng, Assis. Professor, Institute of Information and Communication Technologies, Bulgarian Academy of Science (BAS) 6. PhD (Chemistry), Biotechnology Department, Moscow State University of Applied Biotechnology, Talalikhina Street, 33, Moscow 109316, Russian Federation * E-mail of the corresponding author: gluhchev@iinf.bas.bg Abstract This article outlines the results on the antimicrobial action of electrochemically activated water solutions (anolyte/catholyte). The two types of water solutions are produced in the anode and cathode chamber of the electrolitic cell, respectively. Under laboratory conditions a strain of E. coli DH5, as well as the cell culture and organ suspensions of сlassical swine fever virus (CSF) were treated with the anolyte and the catholyte. By inoculating them with cell cultures the viral presence (the presence of viral antigen) was measured using the immunoperoxidase technique. It was found that anolyte did not affect the growth of the cell culture PK-15; the viral growth in the infection of a cell monolayer with a cell culture CSF virus was affected in the greatest degree by the anolyte in 1:1 dilution and less by other dilutions; whereas the viral growth at the infection of a cell suspension with the cell culture of the CSF virus was affected by the anolyte in dilution 1:1 in the greatest degree, and less by other dilutions; whereas the viral growth at the infection with the CSF in suspension of the cell monolayer was affected by the anolyte in all applied