Int.J.Curr.Microbiol.App.Sci (2019) 8(2): 2219-2223 2218 Original Research Article https://doi.org/10.20546/ijcmas.2019.802.257 Quantification of Biofilm Formation in Indian Isolates of Salmonella enterica Sophia Inbaraj 1 *, Ravi Kant Agrawal 2 , R.K. Agarwal 1 , Prasad Thomas 1 , Manish Kumar 1 , Abhishek 1 and Pallab Choudhury 1 1 Division of Bacteriology and Mycology, 2 Food Microbiology Lab, Livestock Products Technology, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, India *Corresponding author ABSTRACT Introduction Non typhoidal Salmonella sps., is a gram negative bacterial pathogen associated with gastrointestinal tract of farm animals like cattle, pigs and poultry (WHO, 2013). The organism transmits to human via faeco-oral route and causes food borne illness. Non typhoidal salmonellosis outbreaks occur worldwide comprising about 93 million cases of gastroenteritis and 1,55, 000 deaths annually (Majowicz et al., 2010). Various virulence factors of Salmonella sps., which helps in adhesion and invasion are responsible for the pathogenicity. In addition, Salmonella sps., also possess the ability to form biofilms. Biofilms are the aggregates of bacterial species inside an extracellular polysaccharide matrix. Inside biofilms, bacteria will be in natural stationary phase (Lopez et al., 2010) and their physiology will be different from that of their planktonic counterparts. This helps the bacteria to escape from host immune response. It has been studied that Salmonella sps., could able to form biofilms in various surfaces such as plastic, stainless steel, glass, rubber, gall stones, animal cells and plants (Steenackers et al., 2012). The bacteria International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 02 (2019) Journal homepage: http://www.ijcmas.com A total of 60 Salmonella isolates (43 poultry origin, 17 farm animal origin) received from various parts of the country were used in the current study. The objective is to study the biofilm forming potential of the isolates using microtitre plate assay. Study revealed that 38% of the isolates were moderate biofilm producers and 37% were strong biofilm formers. As biofilm contribute to virulence and antimicrobial resistance of the organism, the study remains significant in food safety aspect. Keywords Biofilms, Salmonella, Poultry, Microtitre plates, Farm animals Accepted: 18 January 2019 Available Online: 10 February 2019 Article Info