Research Article Physiological Alterations due to Hepatotoxicity and the Protective Role of Cleome viscosa Linn Seed Extract in Experimental Animals Addai-Mensah Donkor , 1,2 Martin Ntiamoah Donkor , 3 Benjamin Ahenkorah, 4 Kwadwo Asante Asare-Konadu, 5 Emmanuel Asiedu, 5 and Richard Mosobil 6 1 Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, University for Development Studies, Tamale, hana 2 Department of Pharmaceutical Chemistry, School of Pharmacy and Pharmaceutical Sciences, University for Development Studies, Tamale, hana 3 Department of Biochemistry and Forensic Sciences, School of Chemical and Biochemical Sciences, C. K. Tedam University of Technology and Applied Sciences, Navrongo, hana 4 Department of Medical Laboratory Science, Bolgatanga Technical University, Bolgatanga, hana 5 Department of Biochemistry, Faculty of Applied Sciences, University for Development Studies, Tamale, hana 6 Department of Applied Chemistry, School of Chemical and Biochemical Sciences, C. K. Tedam University of Technology and Applied Sciences, Navrongo, hana Correspondence should e addressed to Addai•Mensah Donkor; dr.adonkor@uds.edu.gh and Martin Ntiamoah Donkor; mdonkor@cktutas.edu.gh Received 7 October 2022; Revised 16 November 2022; Accepted 17 November 2022; Published 29 December 2022 Academic Editor: Mustafa Sevindik Copyright©2022Addai•MensahDonkoretal.TisisanopenaccessarticledistriutedundertheCreativeCommonsAttriution License, which permits unrestricted use, distriution, and reproduction in any medium, provided the original work is properly cited. Liver ailment is a key pulic health menace, principally in developing nations. Quite a lot of medicinal forae have een identifed to have liver shielding activities. Te current study was designed to assess in vitro antioxidant and in vivo hepatoprotective activities of seed extracts of Cleome viscosa Linn. (Capparaceae). Phytochemical screening of C. viscosa seed ethanol extract was carried out. Free radical scavenging activity of crude seed extract of the plant was conducted using the DPPH assay method. DNA damage protection potential of the crude seed extract was carried out using extract of the genomic DNA nicking assay. Hepatoprotective activity of the crude seed extract of the plant was carried out ased on CCl 4 •induced liver damage in Wister alino rats. Serum iomarkers (aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and total protein (TP)) were evaluated to fnd out the efect. Histopathological scrutiny was also carried out for all groups of rats to further confrm the discoveries. Te phytochemical screening was positive for alkaloids, favonoids, saponins, steroids, terpenes, tannins, and phenolic compounds in the seed extract. Te antioxidant assay revealed that the ethanol crude extract of C. viscosa exhiited free radical scavenging activity with IC 50 value of 17.82 ± 0.32 μg/mL, and this was further confrmed y the DNA damage protection activity. Pretreatment of the rats with the crude extract of C. viscosa signifcantly reduced ALP (p < 0.05). Te hepatoprotective activity of the seed extract was confrmed y histopathological studies. From this study, it can e concluded that the crude seed extract revealed antioxidant and hepatoprotective activities. For that reason, in the future, oral intake of C. viscosa seed extract as an adjunct natural therapy may e worthwhile to protect against liver failure•mediated inhiitory efects on reproductive function. 1. Introduction Industrial advancement has noxious consequences, and one such example is generation of caron tetrachloride (CCl 4 ) which is a prime potent ecological pollutant. Humans are exposed to caron tetrachloride through oral, inhalation, and dermal routes [1, 2]. Being intoxicated with caron tetrachloride is elieved to e linked with excess free radical production in numerous organs, comprising the liver and kidney [3]. Caron tetrachloride inds to liver cytochrome Hindawi e Scientific World Journal Volume 2022, Article ID 6132201, 11 pages https://doi.org/10.1155/2022/6132201