Online Copy Abstract Aim: Methodology: Results: Interpretation: To detect genetic variants of GPX5 gene and their associations with reproductive and productive traits in the Lumsniang (Hampshire x Niang Megha) pigs. In this study, a total of 87 Lumsniang pigs were selected for collecting blood samples (3-5 ml) from Nucleus Pig Breeding Farm of ICAR Research Complex for North Eastern Hilly region, Umiam, Meghalaya. Genomic DNA was extracted from the blood leukocytes using the Qiagen DNeasy Blood and tissue kits and diluted to working concentration -1 (50 ng μl ) and stored at -20°C, which were used as templates for association of GPX5 variants with polymerase chain reaction. The data for reproductive and productive traits were recorded from all the animals taken in this study. The reproductive and productive traits were analyzed using a general linear model (GLM) procedure of SPSS Version 16.0. Polymorphisms at Intron 1 of the GPX5 gene was determined by PCR-RFLP with HinfI restriction enzyme and DNA sequencing analyses. Two alleles and three genotypes were identified by HinfI digestion of the GPX5 gene. In the SNP-HinfI (g.1896A>G) locus, significant associations were found between GPX5 genotypes and litter size at birth. The genotype AA demonstrated significantly (P<0.05) superior litter size at birth as compared to AG and GG genotypes at the SNP-HinfI locus in Lumsniang pig. The study reported probable associations of GPX5 variants with reproduction and production traits in Lumsniang pig. The genotype AA at locus SNP-HinfI improved the litter size at birth in the studied population. The GPX5 gene may be considered as a DNA marker for marker-assisted selection after validation in a large pig population. Key words: GPX5 gene, Lumsniang pig, Polymorphism, Reproductive traits Productive traits, Novel polymorphisms in GPX5 gene and their association with reproductive and productive traits in Lumsniang (Hampshire x Niang Megha) pigs under subtropical hilly region of Meghalaya 299-305 Vol. 43 2022 March © Triveni Enterprises, Lucknow (India) Received: 20.05.2021 Revised: 29.07.2021 Accepted: 23.10.2021 1 1 1 1 1 1 1 2 1 2 R. Kumar , G. Kadirvel *, G. Khargharia , S. Deori , S. Doley , M. Das , N. Uttam Singh , M. Singh , K.K. Baruah and V.K. Mishra 1 ICAR Research Complex for NEH Region, Umiam- 793 103, India 2 ICAR Research Complex for NEH Region, Nagaland Centre, Medziphema-797 106, India *Corresponding Author Email : velvet.2007@rediffmailcom How to cite : Kumar, R., G. Kadirvel, G. Khargharia, S. Deori, S. Doley, M. Das, N. Uttam Singh, M. Singh, K.K. Baruah and V.K. Mishra: Novel polymorphisms in GPX5 gene and their association with reproductive and productive traits in Lumsniang (Hampshire x Niang Megha) pigs under subtropical hilly region of Meghalaya. J. Environ. Biol., 43, 299-305 (2022). Lumsniang pig Blood sample was collected from each pig using vacuum tubes with EDTA an anticoagulant Genomic DNA was extracted from whole blood using Qiagen DNeasy Blood and Tissue kit Association analyses of the GPX5 gene polymorphism with productive and reproductive traits in pig After PCR-RFLP analysis the samples with different fragments were purified and sequenced PCR amplification was carried out to amplified targeted region of pig GPX5 gene p-ISSN: 0254-8704 • e-ISSN: 2394-0379 • CODEN: JEBIDP Journal website : www.jeb.co.in « E-mail : editor@jeb.co.in Journal of Environmental Biology Triveni Enterprises (Educational Services Set-up) JEB ® DOI : jeb/43/2/MRN-1991 http://doi.org/10.22438/ Original Research Journal of Environmental Biology