Aminy, et al, Pengaruh Induksi Toksin Ubur-ubur (Physalia physalis) terhadap.... Pengaruh Induksi Toksin Ubur-ubur (Physalia physalis) terhadap Gambaran Histopatologi Paru-paru Tikus Wistar (The Influence of Physalia physalis's Toxin Induction on the Histopathological Change of Wistar Rat's Lung) Sayyidah Auliany Aminy, Al Munawir, Erfan Efendi Fakultas Kedokteran Universitas Jember Jln. Kalimantan 37, Jember 68121 e-mail korespondensi: sha_onley@yahoo.com Abstract Abstrak e-Jurnal Pustaka Kesehatan, vol. 2 (No. 3), September 2014 392 Physalia physalis adalah salah satu jenis ubur-ubur beracun yang dapat menimbulkan dyspnea setelah tersengat. Hal ini menunjukkan adanya efek pneumotoxic toksin Physalia physalis. Penelitian ini bertujuan untuk mengetahui efek pneumotoxic protein toksin ubur-ubur Physalia physalis secara in vivo pada tikus Wistar. Penelitian menggunakan sampel tikus galur Wistar jantan dengan berat rata-rata 150 gram dengan 1 kelompok kontrol (K) dan 3 kelompok perlakuan (P), masing-masing kelompok terdiri atas 6 ekor tikus. Kelompok K diinjeksi dengan larutan PZ; P1, P2 dan P3 masing-masing diinjeksi protein toksin ubur-ubur Physalia physalis dengan dosis 10 mg/KgBB, 20 mg/KgBB dan 30 mg/KgBB. Setelah 6 jam organ paru-paru tikus diambil dan direndam dengan formalin 10% dan dilakukan pembuatan preparat histologi, kemudian dilakukan pengamatan secara mikroskopik dan ditentukan tingkat perubahan histopatologi paru-paru dengan menggunakan skor. Hasil penelitian menunjukkan terjadinya perubahan histopatologi pada paru-paru tikus Wistar berupa infiltrasi sel radang, edema paru, degenerasi dan apoptosis sel-sel penyusun paru. Hasil uji statistik Kruskal-wallis menunjukkan data memiliki perbedaan signifikan (p=0,001). Terdapat perbedaan yang signifikan pada perubahan histologi paru-paru antara kelompok K dengan P1, P2 dan P3. Dapat disimpulkan bahwa toksin ubur-ubur Physalia physalis mempengaruhi gambaran histopatologi paru-paru tikus Wistar. Kata kunci: Toksin, ubur-ubur, Physalia physalis, Histopatologi, paru- paru. Physalia physalis is one of poisonous jellyfish which can cause dyspnea after its stung. This fact indicates that Physalia physalis toxin has pneumotoxic activity. This study determined the effect of Physalia physalis toxin on the histopathological change in lungs of Wistar rats. This trials used male Wistar rats with average weight of 150 grams. There were 4 groups, control group (K) and 3 treatment groups (P), each group containing 6 Wistar rats. K group injected with PZ solution; P1, P2 and P3 groups injected with protein of Physalia physalis toxin at dose of 10mg/KgBW, 20 mg/KgBW, and 30 mg/KgBW, respectively. After 6 hours, rats were decapitated, lungs were removed and fixated with 10% formalin for histopathological examination. Microscopic observations was perfomed to determine histopathological change in lungs of Wistar rats by using scores. The result showed that Physalia physalis's toxin induced alteration of histology of lungs in the form of marked infiltration of inflammatory cells, lungs oedema, degeneration and apoptosis of lung constituent cells.The analysis showed that there were significant difference between K and P groups (p=0,001) by Kruskal-Wallis test. There were significant differences between K and P1, P2 and P3 groups. In conclusion, Physalia physalis toxin affected the histopathological change in lungs of Wistar rats. Keywords: Toxin, Jellyfish, Physalia physalis, Histopathology, Lungs.