Please cite this article in press as: Cardoso, V., et al., Temporal restriction of enzyme supplementation in barley-based diets has no effect in broiler performance. Anim. Feed Sci. Tech. (2014), http://dx.doi.org/10.1016/j.anifeedsci.2014.09.007 ARTICLE IN PRESS G Model ANIFEE-13147; No. of Pages 10 Animal Feed Science and Technology xxx (2014) xxx–xxx Contents lists available at ScienceDirect Animal Feed Science and Technology journal homepage: www.elsevier.com/locate/anifeedsci Temporal restriction of enzyme supplementation in barley-based diets has no effect in broiler performance V. Cardoso b,1 , A.P. Ferreira b,1 , M. Costa a,1 , P.I.P. Ponte a , L. Falcão b , J.P. Freire b , M.M. Lordelo b , L.M.A. Ferreira a , C.M.G.A. Fontes a , T. Ribeiro a,∗ a CIISA - Faculdade de Medicina Veterinária, Pólo Universitário do Alto da Ajuda, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal b Instituto Superior de Agronomia, Tapada da Ajuda, 1349-017 Lisboa, Portugal a r t i c l e i n f o Article history: Received 27 February 2014 Received in revised form 1 September 2014 Accepted 12 September 2014 Available online xxx Keywords: Broiler Barley Non-starch polysaccharides Enzyme supplementation a b s t r a c t It is now well established that exogenous microbial -glucanases effectively contribute to improve the nutritive value of barley based diets for broilers while they are predominantly effective in the first stages of the production cycle. Here, the performance of broilers fed on barley-based diets supplemented with a -glucanase enzyme mixture, in part or during the entire length of the production cycle, was evaluated. Six hundred 1-day-old Ross 308 chicks were randomly divided into 4 groups. Animals were fed on a barley-based diet sup- plemented with a commercial enzyme cocktail for the entire duration of the trial (35 days) or exclusively during the first 11 or 23 days of the experiment. Animals of the control group were fed on the basal diet not containing the microbial biocatalyst mixture. Body weight and feed intake were recorded weekly and weight gain and feed conversion ratios were determined. At the end of the trial, 2 animals were slaughtered to collect gastrointesti- nal contents to evaluate viscosity and enzymatic activity. Weight/length of gastrointestinal compartments were also recorded. Data revealed that all -glucanase supplemented groups outperformed non-supplemented birds at day 35. Significant differences in body weight were detected at day 11, suggesting that response to enzyme supplementation occurs, pri- marily, during the initial stages of broiler growth. In addition, final body weight of animals fed on diets supplemented during the first 11 or 23 days of the experiment was identical to animals supplemented during the entire trial (P>0.05). Together the data suggest that in barley-based diets exogenous enzymes act primarily in the earliest periods of broilers growing period and that enzyme supplementation may be restricted to the first 11 days of the production cycle without negatively affecting animal performance. Zymogram anal- ysis allowed detecting -glucanase activity in the crop of non-supplemented birds at day 35. This activity does not arise from endogenous enzymes present in the feed suggest- ing colonization of the crop with a -glucanase secreting microbial population. Although this activity was not sufficient to reduce digesta viscosity in the upper portions of the GI tract when compared with birds exposed during the entire period of the experiment to the exogenous enzymes, it might have contributed to attenuate the antinutritive effects of -glucans at later stages of animal growth. © 2014 Published by Elsevier B.V. ∗ Corresponding author at: Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal Tel.: +351 213652876; fax: +351 213652889. E-mail address: teresaribeiro@fmv.ulisboa.pt (T. Ribeiro). 1 Equal contribution. http://dx.doi.org/10.1016/j.anifeedsci.2014.09.007 0377-8401/© 2014 Published by Elsevier B.V.