Copyright@ Hassan Vatandoost, Mohamad Reza Abai | Biomed J Sci & Tech Res | BJSTR. MS.ID.003536. 15522 Review Article ISSN: 2574 -1241 Effect of Washing on Sustainability of Skintex® Mr Iii Blanket Against Malaria Vector, Anopheles Stephensi Using In Vitro Bioassay Method Hassan Vatandoost 1,2 *, Mohamad Reza Abai 1,2 *, Hemn Yousefi 1 , Ahmad Ali Hanafi Bojd 1,2 , Morteza Zaim 1,2 and Yavar Rassi 1 1 Department of Medical Entomology and Vector Control, School of Public Health, Iran 2 Institute for Environmental Research, School of Public Health, Iran *Corresponding author: Hassan Vatandoost, Department of Medical Entomology and Vector Control, School of Public Health, Iran Mohamad Reza Abai, Institute for Environmental Research, School of Public Health, Tehran, Iran DOI: 10.26717/BJSTR.2019.21.003536 Received: August 24, 2019 Published: August 29, 2019 Citation: Hassan Vatandoost, Mohamad Reza Abai, Hemn Yousefi, Ahmad Ali Hanafi Bojd, Morteza Zaim, et al., Effect of Washing on Sustainability of Skintex® Mr Iii Blanket Against Malaria Vector, Anopheles Stephensi Using In Vitro Bioassay Method. Biomed J Sci & Tech Res 21(1)-2019. BJSTR. MS.ID.003536. ARTICLE INFO Abstract Objective of this study was to evaluate the bioefficacy of Skintex® MR III blanket against main malaria vector, Anopheles stephensi. The effectiveness of blanket was evaluated using conical according to the WHO methods. Washing procedure was followed by WHO method. Results indicated that in Skintex® MR III blanket mortality around 40% in 6 washes and no mortality in control group. After 25 washes the efficacy is reducing. This blanket has more repellency and knockdown effect against malaria vectors. Findings of this study provide guideline for malaria vector control authorities and people using pyrethroid-impregnated blanket especially in the complex emergency conditions. Keywords: Malaria; Blanket; Skintex; Permethrin; Residue Introduction Malaria and other mosquito-borne disease are the major problems worldwide. Currently there are proven and effective tools to fight against malaria including vector control measures [1]. As these tools are scaled up, malaria endemic countries need to continually update the skills and competence of the health workers engaged in malaria control and elimination. Malaria is one of the important infectious diseases in Iran with an average of about 15000 annual cases in the last decade. More than 80% of malaria cases in Iran are reported from three provinces of Sistan and Baluchistan, Hormozgan, and Kerman in southern and southeastern areas of the country. The most routes of malaria cases are immigration from Afghanistan and Pakistan to southern and southeastern areas of the country (Ministry of Health, annual reports). Locally transmitted cases are now concentrated in the south-eastern part of the country, which are affected by extensive population movement across the border with Pakistan, where malaria control faces serious difficulties. In 2009, I.R. Iran set time- bound elimination objectives for its malaria program. There has been excellent progress since, but the continued risk of importation of malaria cases from Pakistan poses a huge challenge, politically, socially, operationally and technically, to malaria elimination in Iran. The situation in the next decade will be absolute elimination or one where a few small short- lived foci emerge from time to time as a result of importation. The latest number of autochthonous cases in the whole country is 42 including 23 local malaria patients, 7 relapsed cases, 12 imported from the other districts by end of July 2016. There are several works on different aspects of malaria including insecticide resistance monitoring [2-12], sibling species, molecular study, new record [13-20], novel methods for vector control [21-26], faunestic study [27,28], use of plants for larval control [29-41] using bednets and long lasting impregnated nets [42-48]morphological studies