22 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ISSN: 2583-4053 Volume-2 Issue-1 || February 2023 || PP. 22-25 https://doi.org/10.55544/jrasb.2.1.5 Journal for Research in Applied Sciences and Biotechnology www.jrasb.com A Study on Genotoxic Potential of Acephate in Clarias batrachus Subhashree Jagyanseni 1 , Saswati Mishra 2 and Satya Narayan Sahoo 3 1 Research Scholar, Department of Life Sciences, GIET University, Gunupur, Odisha, INDIA. 2 Assistant Professor, Department of Bio-Tech/Life Sciences, GIET University, Gunupur, Odisha, INDIA. 3 HOD Zoology, Niali College, Niali, Cuttack, Odisha, INDIA. 1 Corresponding Author: subhashree1995mishra@gmail.com www.jrasb.com || Vol. 2 No. 1 (2023): February Issue Received: 29-12-2022 Revised: 19-01-2023 Accepted: 29-01-2023 ABSTRACT Acephate is an insecticide made up of organophosphates. It is applied to food crops, citrus trees, on golf courses, in commercial or institutional buildings, and as a seed treatment. Products containing acephate can be purchased as tablets, liquids, granules, powders, and water-soluble packs. Acephate 75% brand name-Asataf insecticide manufactured by TATA RALLIS was used for the test. The solvent used was glass double distilled (g.d.d.) water. Fresh water catfish Clarias batrachus were collected from local water bodies of Cuttack district. All the fishes were acclimatized for fifteen days in laboratory aquaria containing 30L dechlorinated tap water prior to the initiation of the experiment. The peripheral blood smear slides were prepared from the blood collected by caudal incision in accordance with Al-Sabti (1986) and Das and Nanda (1986) with some modifications which were prepared animals were sacrificed after 24, 48 and 72 hours of Exposure and were used for each treatment group in both types of administrations (IP and dermal). The increased concentration of acephate directly affects our biological fish sample i.e. Clarias batrachus. Acephate is causing serious problems in fish as per our genotoxicity study of acephate on Clarias batrachus. Clarias batrachus is a commonly found fish species in fresh water habitat which includes ponds, ditches, wetlands and rice fields of India specially in Odisha.The irrational use of pesticides containing acephate in agriculture cause harmful effects on Clarias batrachus, which is a most important species of fish for maintaining the aquatic diversity. Keywords- Acephate, Clarias batrachus, peripheral blood smear, IP, Dermal, genotoxic potential. I. INTRODUCTION Acephate is an insecticide made up of organophosphates. It is applied to food crops, citrus trees, on golf courses, in commercial or institutional buildings, and as a seed treatment. Products containing acephate can be purchased as tablets, liquids, granules, powders, and water-soluble packs. Acephate is categorised by the EPA as a "possible human carcinogen." After receiving acephate as part of their diets for two years, more animals developed liver or adrenal gland tumours. Mice that were fed high doses of acephate all at once had DNA damage in blood cells, although the damage was repaired four days after the exposure. Fish and amphibians are only minimally harmful to acephate. Salamander hatchlings exposed to high concentration of Acephate experienced decreased development, activity, and eating as well as an increase in muscle and spinal column abnormalities. Acute toxic effects of acephate on freshwater fish Puntius sophore are the subject of a 2016 study by Gavit and Patil [1]. Channa punctata's haematological changes were covered by Satish et al. in 2018. due to acephate[2]. According to Shahi& Singh, (2014) pesticides decrease a number of haematological parameters in Clarias batrachus fish, including Hb, RBC, and WBC [3]. Several insecticides have been shown to have genotoxic potential on different freshwater fish by Campana et al. in 1999, Cavas et al. in 2003, and Ismail et al. in 2018 [4,5,6]. The genotoxic potential of acephate in Clarias batrachus was attempted to be shown in this paper.