Plant Archives Vol. 19 No. 2, 2019 pp. 3373-3376 e-ISSN:2581-6063 (online), ISSN:0972-5210 STUDY OF BACTERIALCONTAMINATION IN AL-SHATTRA TEACHING HOSPITAL Mohammed Mousa Atta 1 , Ibtihaj Ahmed kadhim 2 , Adnan Jawad Ahmed 1 , Hatim AJ Al-Shwilly 1 , Haider Q. Baker 1 and Duaa Yahea Talib 1 1 College of Agriculture, University of Sumer, Iraq. 2 College of Education for Girls, University of Thi-Qar, Iraq. Abstract This study carried out in Al-Shattra general hospital in Al-Shattra city and included taking of (48) forty eight samples from different parts of hospital building and health care workers (physicians and assistants) and these samples transported to college of education of girls laboratory by using of culture media. After that culturing of these samples on different culture media and made of different biochemical tests for identification and differentiation of different bacterial spp. Which can cause nosocomial infections. The results showed detection of (13) thirteen different bacterial types and E. coli was predominant at 27.2% the Klebsiella pneumoniaee and Staphylococcus saprophyticus at 22.5% followed by Staphylococcus aureus 20% and the remaining ratio represent other pathogenic bacteria as Pseudomonas aeruginosa and others. Key words : Bacterial contamination, Pseudomonas aeruginosa, Staphylococcus aureus Introduction Bacterial contamination in hospitals in general is higher in repeated using tools and instruments with different highly pathogenic bacterial strains also from well-known strains as normal flora. The members of Enterobacteriaceae and Staphylococcus aureus have the great chance in such places specially those resist to anti biotic as hospital environment (tools and instruments, building parts in general) also, health care workers from the physicians to cleaning worker and chicken workers all those be susceptible to be colonized by different microbial, fungal and yeast strains (Horan et al., 1992). Direct contact with admitted patients or with hospital building in general, tools and instruments has great chance to transport this microbial contaminants which usually lead to high morbidity and mortality to the patients. in American united states there are about 2 million of persons annually acquired nosocomial infection from whom 90000 die. Nosocomial infection is the fifth cause of death in VIP hospitals and in hospitals of developing world countries there are on any evidences or statics due to many causes from which policy some times. In general distribution of nosocomial infection may be estimated in highly economic entrance with 7.6% and in mid economic entrance with 10.1%. from other observations that the bacteria can survive for different times on the surfaces as clothes (gun), stethoscope, sticky tape, computers, key board, electrical key for elevator, mobiles and different inspection tools as blood pressure toll and etc. dangerous of transmission directly proportionate with time of survival of these strains on the surfaces which depend on geographical and environmental conditions as temperature, moisture, organic matter, ability of biofilm formation and methods of sterilization (Tikhomirov, 1987; Ducel et al., 2002). G+ and G- bacteria known with staying for months on the dead surfaces in hospitals and as well-known that the nosocomial infections as MRSA, Pseudomonas aeruginosa, VRE and Acinetobacter baumannii more stable in hospital environment, while in case of some of well-known pathogens as Streptococcus pneumoniae , Streptococcus pyogenes and Haemophilus influenzaee inactivated quickly after dispelling from the patients and have little chance for survival on surfaces. The main cause for such studies is due to its transport directly to the medical staff of hospital in general and to the admitted patients and visitors specifically, as we known this infection lead to increasing of emotional stress for patients and families which may lead to high not restfully and decrease in quality of patient life therefore, there are 5–10 % of patients had nosocomial